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ZIRC
ZFIN ID: ZDB-FIG-170309-17
Wircer et al., 2017 - Homeodomain protein Otp affects developmental neuropeptide switching in oxytocin neurons associated with a long-term effect on social behavior. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 4

Otp paralogs have differential effect on hypothalamic oxt expression.

(AD) Representative high-resolution micrographs of 5 day-old embryos that were subjected to whole mount in situ hybridization with oxt mRNA probe (dorsal view, anterior to the top). oxt-positive neurons in the posterior tuberculum (PT) of otpa−/− mutants are marked by a dotted rectangle (B). Scale bar, 20 µm. (E,F) Box and whisker plots showing OXT cell number counts in the neurosecretory preoptic area (NPO; E) and in the PT (F) in various genotypes. The numbers of embryos used for the quantification are indicated above each box. In the NPO, otpa+/+;otpb+/+ fish differed significantly only from otpa−/−;otpb+/− (*p=0.013) and otpa−/−;otpb−/− (***p=0.000). In the PT otpa+/+;otpb+/+ differed significantly (***p=0.000) only from otpa−/−;otpb+/− and otpa−/−;otpb+/+.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
oxt WT standard conditions Day 5 preoptic area neurosecretory neuron ISH
otpam866/m866 standard conditions Day 5 caudal tuberculum ISH
Day 5 preoptic area neurosecretory neuron ISH
otpam866/m866 ; otpbsa115/sa115 standard conditions Day 5 Not Detected preoptic area neurosecretory neuron ISH
otpbsa115/sa115 standard conditions Day 5 caudal tuberculum ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
otpam866/m866 standard conditions Day 5 caudal tuberculum oxt expression increased amount, abnormal
otpam866/m866 ; otpbsa115/sa115 standard conditions Day 5 preoptic area neurosecretory neuron oxt expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife