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ZFIN ID: ZDB-FIG-160927-18
Robra et al., 2016 - The Intracellular Signaling Molecule Darpp-32 Is a Marker for Principal Neurons in the Cerebellum and Cerebellum-Like Circuits of Zebrafish. Frontiers in Neuroanatomy   10:81 Full text @ Front. Neuroanat.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Term:
Stage Range: Protruding-mouth to Days 7-13

Fig. 10

Darpp-32 expression in the larval optic tectum. Single optical sections of whole mount immunofluorescence stainings. The images are showing one hemisphere of the optic tectum as indicated in the schematic on the right side. (A) Darpp-32 expression in the optic tectum can be first seen at 3 dpf. Darpp-32 is expressed in the neuropil and the cell bodies of projection neurons. (B) Darpp-32 expression in optic tectum at 6 dpf. (C-E) Note that during the course of development the expression of Pvalb7 (D) and Darpp-32 (C) does not overlap in the optic tectum (E). In (C) intense Darpp-32 expression is visible in the superficial most layer, presumably the SM, while Pvalb 7 expression is detectable more closer to the midline, presumably in neurons of the stratum periventriculare (SPV). Darpp-32 positive cell bodies located are more lateral compared to Pvalb 7 positive ones. Scale bar in (A) 20 µm; in (B-E) 50 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ppp1r1b Ab1-ppp1r1b WT standard conditions Protruding-mouth optic tectum IHC
Ab1-ppp1r1b Day 6 optic tectum IHC
Ab1-ppp1r1b Days 7-13 optic tectum IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pvalb IHC WT standard conditions Days 7-13 optic tectum
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in Neuroanatomy for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front. Neuroanat.