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ZFIN ID: ZDB-FIG-160811-31
Antinucci et al., 2016 - A crystal-clear zebrafish for in vivo imaging. Scientific Reports   6:29490 Full text @ Sci. Rep.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Protruding-mouth to Adult

Fig. 1

Generation of crystal, a fully transparent combinatorial pigmentation mutant.

(a) Left: schematic diagram of the main populations of pigment cells in wild type (WT) zebrafish. Centre: cell lineages generating the different populations of pigment cells. Right: mutations affecting genes controlling either pigment cell formation (nacrew2/w2 and roya9/a9) or melanin production (albb4/b4) used to generate the crystal mutant. RPE, retinal pigment epithelium. (b-d) Pigmentation phenotypes of wild type, single mutant, casper and crystal zebrafish at adult (>3 month old, (b)), embryonic (3 dpf, (c)) and larval (7 dpf, (d)) stages. Red dashed boxes indicate crystal mutants. Insets on the right display eye pigmentation phenotypes. 3 dpf and 7 dpf zebrafish treated with 200 ┬ÁM PTU are shown at the bottom of (c,d). Note that the optical transparency of crystal fish is higher than that of wild type, single mutant, casper and PTU-treated fish.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment by environment: N-phenylthiourea Protruding-mouth larval melanophore stripe melanocyte absent, abnormal
Protruding-mouth retinal pigmented epithelium decreased pigmentation, abnormal
Days 7-13 eye has fewer parts of type iridophore, abnormal
Days 7-13 larval melanophore stripe melanocyte absent, abnormal
Days 7-13 retinal pigmented epithelium decreased pigmentation, abnormal
mitfaw2/w2 standard conditions Protruding-mouth eye pigmented, normal
Protruding-mouth larval melanophore stripe melanocyte absent, abnormal
Protruding-mouth retinal pigmented epithelium pigmented, normal
Days 7-13 eye has normal numbers of parts of type iridophore, normal
Days 7-13 larval melanophore stripe melanocyte absent, abnormal
Days 7-13 retinal pigmented epithelium decreased pigmentation, abnormal
Adult eye has normal numbers of parts of type iridophore, normal
Adult melanophore stripe melanocyte absent, abnormal
Adult retinal pigmented epithelium pigmented, normal
mitfaw2/w2; mpv17a9/a9 standard conditions Protruding-mouth eye lacks all parts of type iridophore, abnormal
Protruding-mouth larval melanophore stripe melanocyte absent, abnormal
Protruding-mouth retinal pigmented epithelium pigmented, normal
Days 7-13 eye lacks all parts of type iridophore, abnormal
Days 7-13 larval melanophore stripe melanocyte absent, abnormal
Days 7-13 retinal pigmented epithelium pigmented, normal
Adult eye lacks all parts of type iridophore, abnormal
Adult melanophore stripe melanocyte absent, abnormal
Adult retinal pigmented epithelium pigmented, normal
mpv17a9/a9 standard conditions Protruding-mouth eye lacks all parts of type iridophore, abnormal
Protruding-mouth retinal pigmented epithelium pigmented, normal
Days 7-13 eye lacks all parts of type iridophore, abnormal
Days 7-13 retinal pigmented epithelium pigmented, normal
Adult eye lacks all parts of type iridophore, abnormal
Adult larval melanophore stripe melanocyte decreased amount, abnormal
Adult retinal pigmented epithelium pigmented, normal
slc45a2b4/b4 standard conditions Protruding-mouth larval melanophore stripe melanocyte decreased amount, abnormal
Protruding-mouth retinal pigmented epithelium decreased pigmentation, abnormal
Days 7-13 eye has normal numbers of parts of type iridophore, normal
Days 7-13 larval melanophore stripe melanocyte absent, abnormal
Days 7-13 retinal pigmented epithelium unpigmented, abnormal
Adult eye has normal numbers of parts of type iridophore, normal
Adult melanophore stripe melanocyte absent, abnormal
slc45a2b4/b4; mpv17a9/a9; mitfaw2/w2 standard conditions Protruding-mouth eye lacks parts or has fewer parts of type iridophore, abnormal
Protruding-mouth eye transparent, abnormal
Protruding-mouth larval melanophore stripe melanocyte absent, abnormal
Protruding-mouth retinal pigmented epithelium unpigmented, abnormal
Days 7-13 eye lacks parts or has fewer parts of type iridophore, abnormal
Days 7-13 larval melanophore stripe melanocyte absent, abnormal
Days 7-13 retinal pigmented epithelium decreased pigmentation, abnormal
Adult eye lacks all parts of type iridophore, abnormal
Adult eye transparent, abnormal
Adult melanophore stripe melanocyte absent, abnormal
Adult retinal pigmented epithelium unpigmented, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.