ZFIN ID: ZDB-FIG-160713-4
Lee et al., 2016 - MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment. Scientific Reports   6:27284 Full text @ Sci. Rep.
Anatomical Terms:
Stage Range: 10-13 somites to Pec-fin

Fig. S5

TALEN-mediated knockout of mcrs1 in zebrafish. (A) Alignment of human and zebrafish MCRS1 protein sequence (ClustaIX program). (B) Expression of mcrs1 mRNA during embryonic development was examined by whole-mount in situ hybridization (ss, somite stage; hpf, hours after fertilization). (C) T7E1 cleavage assay for the identification of zebrafish carrying a deletion in mcrs1 gene. Cleaved bands in lane 5 indicates mcrs1 het mutation. (D) Deletion of 11 base pairs in mcrs1 gene exon 4 was confirmed by DNA sequencing. (E) Genotyping of mcrs1 mutants using PCR.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mcrs1 WT standard conditions 10-13 somites whole organism ISH
Prim-15 brain ISH
Prim-15 pronephros ISH
Prim-15 spinal cord ISH
Long-pec brain ISH
Long-pec eye ISH
Pec-fin brain ISH
Pec-fin eye ISH
Antibody Labeling Details No data available
Phenotype Details No data available
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