| ZFIN ID: ZDB-FIG-160713-4 |
Lee et al., 2016
- MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment.
Scientific Reports
6:27284
Full text @ Sci. Rep.
EXPRESSION / LABELING:
| Gene: | |
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| Fish: | |
| Anatomical Terms: | |
| Stage Range: | 10-13 somites to Pec-fin |
Fig. S5 TALEN-mediated knockout of mcrs1 in zebrafish. (A) Alignment of human and zebrafish MCRS1 protein sequence (ClustaIX program). (B) Expression of mcrs1 mRNA during embryonic development was examined by whole-mount in situ hybridization (ss, somite stage; hpf, hours after fertilization). (C) T7E1 cleavage assay for the identification of zebrafish carrying a deletion in mcrs1 gene. Cleaved bands in lane 5 indicates mcrs1 het mutation. (D) Deletion of 11 base pairs in mcrs1 gene exon 4 was confirmed by DNA sequencing. (E) Genotyping of mcrs1 mutants using PCR. |
Gene Expression Details
| Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
|---|---|---|---|---|---|---|
| mcrs1 | WT | standard conditions | 10-13 somites | whole organism | ISH | |
| Prim-15 | brain | ISH | ||||
| Prim-15 | pronephros | ISH | ||||
| Prim-15 | spinal cord | ISH | ||||
| Long-pec | brain | ISH | ||||
| Long-pec | eye | ISH | ||||
| Pec-fin | brain | ISH | ||||
| Pec-fin | eye | ISH |
Antibody Labeling Details
No data available
Phenotype Details
No data available
| Acknowledgments: | This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep. |
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