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Fig. S5
TALEN-mediated knockout of mcrs1 in zebrafish. (A) Alignment of human and zebrafish MCRS1 protein sequence (ClustaIX program). (B) Expression of mcrs1 mRNA during embryonic development was examined by whole-mount in situ hybridization (ss, somite stage; hpf, hours after fertilization). (C) T7E1 cleavage assay for the identification of zebrafish carrying a deletion in mcrs1 gene. Cleaved bands in lane 5 indicates mcrs1 het mutation. (D) Deletion of 11 base pairs in mcrs1 gene exon 4 was confirmed by DNA sequencing. (E) Genotyping of mcrs1 mutants using PCR.
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