ZFIN ID: ZDB-FIG-160713-5
Lee et al., 2016 - MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment. Scientific Reports   6:27284 Full text @ Sci. Rep.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. S6

Phenotype of homozygous mcrs1 mutant zebrafish. (A) Homozygous mcrs1 mutants showed increased apoptotic cell death in the central nervous system. Apoptotic cells were detected by acridine orange staining at 48 and 72 hpf. Insets are magnified view of the head. (B) Injection of mcrs1 mRNA rescued excessive apoptotic cell death in mcrs1 mutant zebrafish. (C) The retinal sections from embryos at 48 and 60 hpf were stained with anti-active caspase3 antibody. (D) The retinal sections from embryos at 48 hpf were stained with anti-phospho-histone H3 antibody. (E) Whole-mount in situ hybridization of cmlc2. The establishment of left/right body axis is normal in homozygous mcrs1 mutants.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mcrs1ck018/ck018 standard conditions Long-pec central nervous system apoptotic, abnormal
Long-pec left/right axis specification process quality, normal
Long-pec neurogenesis disrupted, abnormal
Long-pec retina apoptotic, abnormal
Protruding-mouth central nervous system apoptotic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.