header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-151214-68
Askary et al., 2015 - Iroquois Proteins Promote Skeletal Joint Formation by Maintaining Chondrocytes in an Immature State. Developmental Cell   35:358-65 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-25 to Days 14-20
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 1

Regulation of irx7 and irx5a Expression and Character of the Hyoid Joint

(A and B) Schematics of mandibular and hyoid arches at 36 hpf and cartilage derivatives at 5 dpf show early regulation of joints by Hand2 and Edn1 and late regulation by Bmp signaling.

(C) In situ hybridization at 36 hpf shows expression of irx7 (red) relative to dlx2a+ neural-crest-derived cells (green) in a domain of intermediate hyoid arch cells (dashed line) and in a portion of the first pharyngeal pouch (arrow).

(D) Relative to sox10:GFPCAAX+ cartilages (anti-GFP, blue), overlapping expression of irx7 (red) and irx5a (green) is seen at the developing hyoid joint.

(E and F) An irx7:GFP gene-trap line (green) shows increasingly restricted expression at the bipartite hyoid joint (arrows) relative to sox10:mCherryCAAX+ cartilages (red).

(G) trps1:GFP labels a similar population of cells as irx7 at each side of the hyoid joint (arrows) relative to sox10:dsRed+ cartilages.

(H) Trichrome staining shows that cells at the hyoid joints (arrows) are smaller and lack the large lacunae of neighboring hypertrophic chondrocytes. Black line denotes merging of two adjacent sections.

(I–T) Expression of irx7 and irx5a (red) relative to sox9a+ hyoid chondrocytes (green) in edn1, hand2, and moz mutants, as well as hsp70I:Gal4 control and hsp70I:Gal4; UAS:Bmp4 embryos subjected to 40–44 hpf heat shock. Brackets show ventral expansion in hand2 mutants. M, Meckel’s; Pq, palatoquadrate; Sy, symplectic; Hm, hyomandibula; Ch, ceratohyal; Ih, interhyal. Numbers indicate proportion of animals showing the displayed phenotype.

Scale bars represent 30 µM. See also Figure S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a WT standard conditions Prim-25 pharyngeal arch 1 mesenchymal cell ISH
Prim-25 pharyngeal arch 2 mesenchymal cell ISH
DsRed el10Tg ; trps1j1271aGt standard conditions Days 14-20 ceratohyal cartilage chondrocyte IFL
Days 14-20 hyosymplectic cartilage chondrocyte IFL
EGFP el10Tg ; trps1j1271aGt standard conditions Days 14-20 ceratohyal-interhyal joint IFL
Days 14-20 interhyal-hyosymplectic joint IFL
el375Tg standard conditions Long-pec pharyngeal arch 2 mesenchymal cell IFL
irx7nksagp011Gt ; el361Tg standard conditions Day 5 pharyngeal arch 2 skeleton joint IFL
Days 14-20 ceratohyal-interhyal joint IFL
Days 14-20 interhyal-hyosymplectic joint IFL
irx5a Df(Chr01:hand2)s6/s6 standard conditions Long-pec pharyngeal arch 2 joint ISH
WT standard conditions Long-pec pharyngeal arch 2 joint ISH
edn1tf216b/tf216b standard conditions Long-pec Not Detected pharyngeal arch 2 joint ISH
el375Tg standard conditions Long-pec pharyngeal arch 2 joint ISH
el49Tg; kca4Tg heat shock Long-pec Not Detected pharyngeal arch 2 joint ISH
kat6ab719/b719 standard conditions Long-pec Not Detected pharyngeal arch 2 joint ISH
kca4Tg heat shock Long-pec pharyngeal arch 2 joint ISH
irx7 Df(Chr01:hand2)s6/s6 standard conditions Long-pec pharyngeal arch 2 joint ISH
WT standard conditions Prim-25 pharyngeal arch 2 mesenchymal cell ISH
Prim-25 pharyngeal pouch 1 ISH
Long-pec pharyngeal arch 2 joint ISH
edn1tf216b/tf216b standard conditions Long-pec Not Detected pharyngeal arch 2 joint ISH
el375Tg standard conditions Long-pec pharyngeal arch 2 joint ISH
el49Tg; kca4Tg heat shock Long-pec Not Detected pharyngeal arch 2 joint ISH
kat6ab719/b719 standard conditions Long-pec Not Detected pharyngeal arch 2 joint ISH
kca4Tg heat shock Long-pec pharyngeal arch 2 joint ISH
mCherry irx7nksagp011Gt ; el361Tg standard conditions Day 5 ceratohyal cartilage chondrocyte IFL
Day 5 hyosymplectic cartilage chondrocyte IFL
Days 14-20 ceratohyal cartilage chondrocyte IFL
Days 14-20 hyosymplectic cartilage chondrocyte IFL
sox9a Df(Chr01:hand2)s6/s6 standard conditions Long-pec pharyngeal arch 2 chondrocyte ISH
WT standard conditions Long-pec pharyngeal arch 2 chondrocyte ISH
edn1tf216b/tf216b standard conditions Long-pec pharyngeal arch 2 chondrocyte ISH
el49Tg; kca4Tg heat shock Long-pec pharyngeal arch 2 chondrocyte ISH
kat6ab719/b719 standard conditions Long-pec pharyngeal arch 2 chondrocyte ISH
kca4Tg heat shock Long-pec pharyngeal arch 2 chondrocyte ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
Df(Chr01:hand2)s6/s6 standard conditions Long-pec pharyngeal arch 2 joint irx7 expression increased distribution, abnormal
Long-pec pharyngeal arch 2 joint irx5a expression increased distribution, abnormal
edn1tf216b/tf216b standard conditions Long-pec pharyngeal arch 2 joint irx5a expression absent, abnormal
Long-pec pharyngeal arch 2 joint irx7 expression absent, abnormal
el49Tg; kca4Tg heat shock Long-pec pharyngeal arch 2 joint irx5a expression absent, abnormal
Long-pec pharyngeal arch 2 joint irx7 expression absent, abnormal
kat6ab719/b719 standard conditions Long-pec pharyngeal arch 2 joint irx7 expression absent, abnormal
Long-pec pharyngeal arch 2 joint irx5a expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 35, Askary, A., Mork, L., Paul, S., He, X., Izuhara, A.K., Gopalakrishnan, S., Ichida, J.K., McMahon, A.P., Dabizljevic, S., Dale, R., Mariani, F.V., Crump, J.G., Iroquois Proteins Promote Skeletal Joint Formation by Maintaining Chondrocytes in an Immature State, 358-65, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell