ZFIN ID: ZDB-FIG-151130-8
Kiesow et al., 2015 - Junb controls lymphatic vascular development in zebrafish via miR-182. Scientific Reports   5:15007 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 6 Failure in TD formation of Junb morphants is restored by ectopic miR-182.

(a) Left panel, confocal images of trunk vasculature of representative larvae at 5 dpf injected with indicated morpholino or mimic: std-MO (4 ng, n = 62), junba-MO1 + junbb-MO1 (2 ng + 2 ng, n = 60), miR-182-5MM-MO (12 ng, n = 59), miR-182-MO (12 ng, n = 60), junba-MO1 + junbb-MO1 + 182-Mimic (2 ng + 2 ng + 40 pg, n = 32). Middle panel, confocal images of indicated trunk region. Right panel, enlarged view of trunk region marked by yellow rectangle in the middle panel. TD is located between dorsal aorta (DA) and posterior cardinal vein (PCV) and is marked with yellow arrows or if absent with asterisks. Panels are lateral view; dorsal is up, anterior to the left. White scale bar: 50 ┬Ám. (b,c) Percentage of TD defects in larvae for the first (b) and the second set of junb-MOs (c). (d) Left panel, confocal images of trunk vasculature of representative larvae at 5 dpf CRISPR gRNA-injected larvae with indicated junb-CRISPR gRNAs or control CRISPR gRNA: junba-CRISPR (250 pg gRNA), junbb-CRISPR (250 pg gRNA), junba-CRISPR + junbb-CRISPR (200 pg + 200 pg gRNA) and Co-CRISPR (400 pg gRNA). Labelling and size bar as described in (a). (e) Percentage of TD defects in larvae at 5 dpf injected with CRISPR gRNAs either against junba, junbb or a combination of both versus larvae injected with control CRISPR gRNA. Sum of 10 somite segments per embryo was categorized into groups with presence, partial or complete absence of TD. ***P < 0.001, Mann-Whitney U-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Day 5 dorsal aorta IFL
Day 5 dorsal longitudinal anastomotic vessel IFL
Day 5 intersegmental vessel IFL
Day 5 posterior cardinal vein IFL
Day 5 thoracic duct IFL
y1Tg + CRISPR1-junba control Day 5 dorsal aorta IFL
Day 5 dorsal longitudinal anastomotic vessel IFL
Day 5 intersegmental vessel IFL
Day 5 posterior cardinal vein IFL
y1Tg + CRISPR1-junba + CRISPR1-junbb control Day 5 dorsal aorta IFL
Day 5 dorsal longitudinal anastomotic vessel IFL
Day 5 intersegmental vessel IFL
Day 5 posterior cardinal vein IFL
y1Tg + CRISPR1-junbb control Day 5 dorsal aorta IFL
Day 5 dorsal longitudinal anastomotic vessel IFL
Day 5 intersegmental vessel IFL
Day 5 posterior cardinal vein IFL
y1Tg + MO2-junba + MO3-junbb + MO4-tp53 control Day 5 dorsal aorta IFL
Day 5 dorsal longitudinal anastomotic vessel IFL
Day 5 intersegmental vessel IFL
Day 5 posterior cardinal vein IFL
y1Tg + MO3-mir182 + MO4-tp53 control Day 5 dorsal aorta IFL
Day 5 dorsal longitudinal anastomotic vessel IFL
Day 5 intersegmental vessel IFL
Day 5 posterior cardinal vein IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + CRISPR1-junba control Day 5 lymph vessel development arrested, abnormal
Day 5 thoracic duct absent, abnormal
y1Tg + CRISPR1-junba + CRISPR1-junbb control Day 5 lymph vessel development arrested, abnormal
Day 5 thoracic duct absent, abnormal
y1Tg + CRISPR1-junbb control Day 5 lymph vessel development arrested, abnormal
Day 5 thoracic duct absent, abnormal
y1Tg + MO2-junba + MO3-junbb + MO4-tp53 control Day 5 lymph vessel development arrested, abnormal
Day 5 thoracic duct absent, abnormal
y1Tg + MO3-mir182 + MO4-tp53 control Day 5 lymph vessel development arrested, abnormal
Day 5 thoracic duct absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.