ZFIN ID: ZDB-FIG-151130-7
Kiesow et al., 2015 - Junb controls lymphatic vascular development in zebrafish via miR-182. Scientific Reports   5:15007 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 5 Ectopic miR-182 restores PL formation in Junb morphants.

(a) Left panel, bright field images showing overall morphology of junba and junbb (2 + 2 ng) double morphants injected with or without Neg-Mimic (40 pg, n = 127) or 182-Mimic (40 pg, n = 109). Middle panel: Confocal images of indicated trunk region in Tg(fli1:EGFP)y1 embryos at 72 hpf. Right panel, enlarged view of trunk region marked by the yellow rectangle in the middle panel. DA, dorsal aorta, PCV, posterior cardinal vein and ISV, intersegmental vessels. PL is marked with yellow arrows or if absent with asterisks. Panels are lateral view; dorsal is up, anterior to the left. Scale bars: 500 µm (black); 50 µm (white). (b) Percentage of embryos injected with MOs and Mimics as indicated and (c) for a second set of junb-MOs: junba-MO2 + junbb-MO2 (2 ng + 2 ng, n = 112), with or without Neg-Mimic (40 pg, n = 145) or 182-Mimic (40 pg, n = 119). ***P < 0.001, Mann-Whitney U-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg control Protruding-mouth dorsal aorta IFL
Protruding-mouth dorsal longitudinal anastomotic vessel IFL
Protruding-mouth intersegmental vessel IFL
Protruding-mouth posterior cardinal vein IFL
Protruding-mouth vascular lymphangioblast IFL
y1Tg + MO2-junba + MO3-junbb + MO4-tp53 control Protruding-mouth dorsal aorta IFL
Protruding-mouth dorsal longitudinal anastomotic vessel IFL
Protruding-mouth intersegmental vessel IFL
Protruding-mouth posterior cardinal vein IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO2-junba + MO3-junbb + MO4-tp53 control Long-pec vascular lymphangioblast absent, abnormal
Protruding-mouth vascular lymphangioblast absent, abnormal
y1Tg + MO3-junba + MO4-junbb + MO4-tp53 control Long-pec vascular lymphangioblast absent, abnormal
Protruding-mouth vascular lymphangioblast absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.