ZFIN ID: ZDB-FIG-151130-34
Swindell et al., 2015 - Crispld2 is required for neural crest cell migration and cell viability during zebrafish craniofacial development. Genesis (New York, N.Y. : 2000)   53(10):660-7 Full text @ Genesis
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 1

NCC migration is disorganized in MO3 morphants. Time-lapse live cell imaging captures of sox10:GFP embryos showing migrating NCC cells at 16 (a, b), 22 (c, d), and 28 hpf (e, f). UIC (a, c, and e) and MO3-injected embryos (b, d, and f) showing a dorsal view of migrating NCCs. White arrows point to abnormally migrating NCCs. (g) Quantification of number of cells crossing the midline in UIC, MO3 and control MM-MO3 injected embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ba2Tg standard conditions 14-19 somites neural crest cell IFL
26+ somites neural crest cell IFL
Prim-5 neural crest cell IFL
ba2Tg + MO3-crispld2 standard conditions 14-19 somites neural crest cell IFL
26+ somites neural crest cell IFL
Prim-5 neural crest cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ba2Tg + MO3-crispld2 standard conditions 14-19 somites - Prim-5 neural crest cell migration process quality, abnormal
14-19 somites - Prim-5 whole organism has fewer parts of type neural crest cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Genesis (New York, N.Y. : 2000) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Genesis