ZFIN ID: ZDB-FIG-150423-42
Windner et al., 2015 - Tbx6, Mesp-b and Ripply1 regulate the onset of skeletal myogenesis in zebrafish. Development (Cambridge, England)   142(6):1159-68 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 5

Ripply1 knockdown inhibits fast muscle development, dermomyotome maturation is delayed. Control and Ripply1 morphants at the 20-somite (A,B), 22-somite (C,D) and 24-h stage (E-K). (A,B) Ripply1 morphants show upregulation of meox1 (A) and downregulation of pax3 (B) mRNA. (C,D) In control embryos, Tbx6 is restricted to pre-somitic mesoderm, Pax7+ dermomyotome cells populate somites 1-18; in Ripply1 morphants, Tbx6 protein is expanded into the posterior trunk, Pax7+ cells are restricted to somites <7-14 (D). The Pax7 and Tbx6 expression domains (green and cyan lines, respectively) do not overlap; the dorsal and ventral domains of the trunk develop faster than the central domain (arrowheads). (E-G) At 24h, tbx6 mRNA expression is only slightly expanded in Ripply1 morphants (E); pax3 (F) and pax7 (G) are highly upregulated. (H,I) Immunolabeling shows an increase in Pax7+ dermomyotome cells in Ripply1 morphants. Boxes separately show Mef2+ (red) and Pax7+ nuclei (green, number indicated). (J,K) In wild type, slow muscle fibers (F59, cyan) are found lateral to fast fibers; Ripply1 morphants completely lack fast fibers (red, on cross-sections in J,K), slow fibers lie adjacent to the axial structures. β-catenin labels cell membranes, Hoechst all nuclei. Axis levels of images in H-K are indicated in G. Scale bars: 100µm in B,D,G; 50µm in I,K.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
meox1 WT control 20-25 somites somite ISH
WT + MO1-ripply1 + MO4-tp53 control 20-25 somites somite ISH
pax3a WT control 20-25 somites somite ISH
Prim-5 trunk ISH
WT + MO1-ripply1 + MO4-tp53 control 20-25 somites somite ISH
Prim-5 trunk ISH
pax7a WT control Prim-5 trunk ISH
WT + MO1-ripply1 + MO4-tp53 control Prim-5 trunk ISH
tbx6 Ab2-tbx6 WT control 20-25 somites segmental plate IHC
Prim-5 somite ISH
Prim-5 trunk posterior region ISH
Ab2-tbx6 WT + MO1-ripply1 + MO4-tp53 control 20-25 somites segmental plate IHC
Ab2-tbx6 20-25 somites trunk posterior region IHC
Prim-5 somite ISH
Prim-5 trunk posterior region ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC WT control Prim-5 slow muscle cell
IHC WT + MO1-ripply1 + MO4-tp53 control Prim-5 slow muscle cell
Ab-Pax7 IHC WT control 20-25 somites trunk
IHC Prim-5 dermomyotome
IHC WT + MO1-ripply1 + MO4-tp53 control 20-25 somites trunk
IHC Prim-5 dermomyotome
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ripply1 + MO4-tp53 control 20-25 somites somite pax3a expression decreased amount, abnormal
WT + MO1-ripply1 + MO4-tp53 control 20-25 somites somite meox1 expression increased amount, abnormal
WT + MO1-ripply1 + MO4-tp53 control 20-25 somites trunk ab-pax7 labeling increased distribution, abnormal
WT + MO1-ripply1 + MO4-tp53 control 20-25 somites trunk posterior region tbx6 expression mislocalised, abnormal
WT + MO1-ripply1 + MO4-tp53 control Prim-5 dermomyotome increased amount, abnormal
WT + MO1-ripply1 + MO4-tp53 control Prim-5 slow muscle cell mislocalised, abnormal
WT + MO1-ripply1 + MO4-tp53 control Prim-5 trunk pax7a expression increased distribution, abnormal
WT + MO1-ripply1 + MO4-tp53 control Prim-5 trunk pax3a expression increased distribution, abnormal
WT + MO1-ripply1 + MO4-tp53 control Prim-5 trunk posterior region tbx6 expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development