ZFIN ID: ZDB-FIG-150423-40
Windner et al., 2015 - Tbx6, Mesp-b and Ripply1 regulate the onset of skeletal myogenesis in zebrafish. Development (Cambridge, England)   142(6):1159-68 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 10-13 somites to Prim-5

Fig. 3

Mesp-baMyc inhibits fast fiber differentiation and promotes dermomyotome development. Tg(hsp70l:mesp-bamyc) embryos and wild-type siblings 1h (A-D), 6h (24-somite stage) (E-H) and 9h (24-h stage) (I,J) after the end of heat shock. (A-D) Transgenic embryos show downregulation of myoD (A) and myf5 mRNA (B) in the lateral paraxial mesoderm, and upregulation of meox1 (C), but no difference in pax3 (D). Most recently formed somite SI is outlined. (E,F) myoD expression shows smaller myotomes (E, brackets) in somites formed after heat shock (orange arrowhead); meox1 mRNA is upregulated throughout those somites (F, brackets). (G,H) In transgenic embryos (Myc+, gray), the number of slow muscle nuclei (Mef2, bright red) is similar to wild type, but fast fiber nuclei (Mef2, faint red) and myosin heavy chain protein (MF20, blue) are reduced (arrowheads). (I,J) Immunolabeling shows increase in Pax7+ dermomyotome cells (green) in Tg(hsp70l:mesp-bamyc) embryos (number indicated); boxed areas show underlying myotomes (MF20, blue) on the right. Orange arrowheads mark last somite formed before heat shock. Scale bars: 100µm in D,F; 50µm in H,J.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
meox1 WT control 10-13 somites paraxial mesoderm ISH
20-25 somites somite ISH
zf605Tg heat shock 10-13 somites paraxial mesoderm ISH
20-25 somites somite ISH
myf5 WT control 10-13 somites paraxial mesoderm lateral region ISH
zf605Tg heat shock 10-13 somites paraxial mesoderm lateral region ISH
myod1 WT control 10-13 somites paraxial mesoderm lateral region ISH
20-25 somites somite ISH
Prim-5 myotome ISH
zf605Tg heat shock 10-13 somites paraxial mesoderm lateral region ISH
20-25 somites somite ISH
Prim-5 myotome ISH
pax3a WT control 10-13 somites paraxial mesoderm ISH
zf605Tg heat shock 10-13 somites paraxial mesoderm ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT control 26+ somites myotome
IHC zf605Tg heat shock 26+ somites myotome
Ab-Pax7 IHC WT control Prim-5 dermomyotome
IHC zf605Tg heat shock Prim-5 dermomyotome
Phenotype Details
Fish Conditions Stage Phenotype
zf605Tg heat shock 10-13 somites paraxial mesoderm meox1 expression increased amount, abnormal
zf605Tg heat shock 10-13 somites paraxial mesoderm lateral region myf5 expression decreased amount, abnormal
zf605Tg heat shock 10-13 somites paraxial mesoderm lateral region myod1 expression decreased amount, abnormal
zf605Tg heat shock 20-25 somites myotome decreased size, abnormal
zf605Tg heat shock 20-25 somites somite meox1 expression increased distribution, abnormal
zf605Tg heat shock 26+ somites fast muscle cell decreased amount, abnormal
zf605Tg heat shock 26+ somites myotome ab-mf20 labeling decreased amount, abnormal
zf605Tg heat shock Prim-5 dermomyotome increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development