Fig. 2

Gu et al., 2015 - A Novel TGFβ Modulator that Uncouples R-Smad/I-Smad-Mediated Negative Feedback from R-Smad/Ligand-Driven Positive Feedback
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

Knockdown of ldb2a enhances Nodal/BMP signal transduction in zebrafish embryos.

(A) At the onset of gastrulation, ldb2a expression is more noticeable in the yolk syncytial layer (YSL) (white arrowheads), which is an important source of Nodal signals (purple arrows). (B) At the shield stage, western blot showed increased phosphorylation of R-Smad2 in ldb2a morphants, while the level of overall Smad2/3 remained the same. β-actin was used as a loading control. (C) The activity of a TGFβ reporter SBE-luciferase was up-regulated in shield stage ldb2a morphants. (D) Phosphorylation of R-Smad1/5/8 was up-regulated in ldb2a morphants at the tailbud stage. β-tubulin was the loading control. (E) The activity of a BMP reporter Id1-BRE2-luciferase was increased in tailbud stage ldb2a morphants. (C and E) each displays a single representative experiment of three biological replicates, with the error bars corresponding to two technical replicates. For each biological replicate, 50 embryos were lysed and analysed. (F-I′) Expression of Nodal ligands, cyc and sqt, was increased in shield stage ldb2a morphants. (J-K′) Anterior and posterior expression of bmp4 was up-regulated at the tailbud stage. (F-G′): seven independent experiments, with the total number of analysed embryos shown on the top-right corner of each panel; (H-K′): two independent experiments. The wildtype control refers to uninjected embryos that are stage matched.

Expression Data
Knockdown Reagents:
Anatomical Terms:
Stage Range: Shield to Bud

Expression Detail
Antibody Labeling
Phenotype Data
Knockdown Reagents:
Observed In:
Stage Range: Shield to Bud

Phenotype Detail
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.