ZFIN ID: ZDB-FIG-150327-2
Glenn et al., 2014 - Distinct regulation of the anterior and posterior myeloperoxidase expression by Etv2 and Gata1 during primitive Granulopoiesis in zebrafish. Developmental Biology   393(1):149-59 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Fig. 2

Comparison of mpx, etv2, scl, gata1, hbae3, etv2:GFP and etv2:Kaede expression. (A–L) mpx expression overlaps with scl, gata1 and hbae3, but not etv2, in the ICM of wild-type embryos at the 22-somite stage, as analyzed by two color in situ hybridization. (A–C) etv2 (arrows), and mpx (arrowheads) expression domains largely do not overlap. Maximum intensity projections of confocal images are shown. Autofluorescent yolk granules are apparent in the lower left corner in (B and C). (D–F) mpx expression partially overlaps with scl expression (selected overlapping cells, arrowheads). Single confocal slices are shown. (G–I) mpx and gata1 expression largely overlap (arrowheads, selected cells). Single confocal slices are shown. (J–L) mpx and hbae3 expression largely overlap (arrowheads, selected cells). Note that not all hbae3 cells are positive for mpx expression. Single confocal slices are shown. (M–O) Only a fraction (10.8%) of mpx-expressing cells in the ICM overlap with etv2:GFP expression at the 24 somite stage, as observed by combined ISH for mpx expression and immunohistochemistry for GFP. (P-R) etv2:Kaede and mpx:GFP expression overlaps in neutrophils (white arrowheads) in the trunk ICM region of embryos at 24 hpf. Yellow arrows denote vascular endothelial cells. (S–U) Multiple (64%) ALPM-derived mpx-expressing cells, positioned over the yolk, overlap with etv2:GFP expression at 24 hpf (white arrowheads) as observed by combined ISH for mpx expression (red) and immunohistochemistry for GFP (green). (S′–U′′) Magnified views of individual cells from S–U. Images show anterior to the left and dorsal up in all panels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ci1Tg standard conditions 20-25 somites intermediate cell mass of mesoderm IHC
Prim-5 anterior lateral plate mesoderm IHC
etsrp WT standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
gata1a WT standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
GFP ci6Tg; uwm1Tg standard conditions Prim-5 neutrophil IHC
hbae3 WT standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
Kaede ci6Tg; uwm1Tg standard conditions Prim-5 blood vessel endothelial cell IHC
Prim-5 neutrophil IHC
mpx WT standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
ci1Tg standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
Prim-5 anterior lateral plate mesoderm ISH
tal1 WT standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 393(1), Glenn, N.O., Schumacher, J.A., Kim, H.J., Zhao, E.J., Skerniskyte, J., Sumanas, S., Distinct regulation of the anterior and posterior myeloperoxidase expression by Etv2 and Gata1 during primitive Granulopoiesis in zebrafish, 149-59, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.