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ZFIN ID: ZDB-FIG-150224-18
Kjaer-Sorensen et al., 2014 - Papp-a2 modulates development of cranial cartilage and angiogenesis in zebrafish embryos. Journal of Cell Science   127(23):5027-37 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 7

papp-a2-knockdown reduces Notch signaling activity in the CNS. (A) Representative images of control and papp-a2-knockdown Notch activity reporter embryos [Tg(Tp1bglob:eGFP)um13] showing the range of Notch reporter fluorescence levels. Embryos were grouped according to fluorescence levels ranging from 1 (very low Notch signaling activity) to 4 (maximum Notch signaling activity). (B) Plot showing the percentage of embryos at each fluorescence level in control and knockdown embryos. n = 20 (cMO), 24 (e1i1), 30 (ATG), 14 (i3e4). (C) her6 whole-mount in situ hybridization of 24 hpf embryos. Numbers indicate fractions of embryos displaying the depicted phenotype. hb, hindbrain; mb, midbrain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
her6 WT control Prim-5 hindbrain ISH
Prim-5 midbrain ISH
WT + MO1-pappa2 + MO4-tp53 standard conditions Prim-5 hindbrain ISH
Prim-5 midbrain ISH
WT + MO2-pappa2 + MO4-tp53 standard conditions Prim-5 hindbrain ISH
Prim-5 midbrain ISH
WT + MO3-pappa2 + MO4-tp53 standard conditions Prim-5 hindbrain ISH
Prim-5 midbrain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
um13Tg + MO1-pappa2 + MO4-tp53 standard conditions Long-pec Notch signaling pathway disrupted, abnormal
um13Tg + MO2-pappa2 + MO4-tp53 standard conditions Long-pec Notch signaling pathway disrupted, abnormal
um13Tg + MO3-pappa2 + MO4-tp53 standard conditions Long-pec Notch signaling pathway disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.