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ZFIN ID: ZDB-FIG-150224-16
Kjaer-Sorensen et al., 2014 - Papp-a2 modulates development of cranial cartilage and angiogenesis in zebrafish embryos. Journal of Cell Science   127(23):5027-37 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 4

Papp-a2 is required for normal development of cranial cartilages. (A) Standard light microscopy images of papp-a2-knockdown and control embryos showing severely reduced jaw development. Lateral views, anterior to the left. (B) staining of papp-a2 knockdown and control embryos showing reduction or absence of the ethmoid (e), trabecula cranii (t), Meckel′s cartilage (m), palatoquadrate (pq), ceratohyal (ch), hyosymplectic (hs), and the five caudal branchial arches (*). Nc, notochord. (C) Flat-mounted 24 hpf zebrafish embryos whole mount in situ stained for dlx2a. Numbers indicate the fraction of embryos displaying the depicted phenotype. Dorsal view, anterior to the left. Arrowheads, clusters of dlx2a-positive postmigratory cranial neural cells.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a WT control Prim-5 cranial neural crest cell ISH
WT + MO1-pappa2 + MO4-tp53 standard conditions Prim-5 cranial neural crest cell ISH
WT + MO2-pappa2 + MO4-tp53 standard conditions Prim-5 cranial neural crest cell ISH
WT + MO3-pappa2 + MO4-tp53 standard conditions Prim-5 cranial neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-pappa2 + MO4-tp53 standard conditions Prim-5 cranial neural crest cell decreased amount, abnormal
Protruding-mouth ceratohyal cartilage absent, abnormal
Protruding-mouth ceratohyal cartilage decreased size, abnormal
Protruding-mouth ethmoid cartilage decreased size, abnormal
Protruding-mouth hyosymplectic cartilage absent, abnormal
Protruding-mouth hyosymplectic cartilage decreased size, abnormal
Protruding-mouth Meckel's cartilage absent, abnormal
Protruding-mouth Meckel's cartilage decreased size, abnormal
Protruding-mouth neurocranial trabecula absent, abnormal
Protruding-mouth neurocranial trabecula decreased size, abnormal
Protruding-mouth palatoquadrate arch absent, abnormal
Protruding-mouth palatoquadrate arch decreased size, abnormal
Protruding-mouth pharyngeal arch 3-7 decreased size, abnormal
WT + MO2-pappa2 + MO4-tp53 standard conditions Prim-5 cranial neural crest cell decreased amount, abnormal
Protruding-mouth ceratohyal cartilage absent, abnormal
Protruding-mouth ceratohyal cartilage decreased size, abnormal
Protruding-mouth ethmoid cartilage decreased size, abnormal
Protruding-mouth hyosymplectic cartilage absent, abnormal
Protruding-mouth hyosymplectic cartilage decreased size, abnormal
Protruding-mouth Meckel's cartilage absent, abnormal
Protruding-mouth Meckel's cartilage decreased size, abnormal
Protruding-mouth neurocranial trabecula absent, abnormal
Protruding-mouth neurocranial trabecula decreased size, abnormal
Protruding-mouth palatoquadrate arch absent, abnormal
Protruding-mouth palatoquadrate arch decreased size, abnormal
Protruding-mouth pharyngeal arch 3-7 decreased size, abnormal
WT + MO3-pappa2 + MO4-tp53 standard conditions Prim-5 cranial neural crest cell decreased amount, abnormal
Protruding-mouth ceratohyal cartilage absent, abnormal
Protruding-mouth ceratohyal cartilage decreased size, abnormal
Protruding-mouth ethmoid cartilage decreased size, abnormal
Protruding-mouth hyosymplectic cartilage absent, abnormal
Protruding-mouth hyosymplectic cartilage decreased size, abnormal
Protruding-mouth Meckel's cartilage absent, abnormal
Protruding-mouth Meckel's cartilage decreased size, abnormal
Protruding-mouth neurocranial trabecula absent, abnormal
Protruding-mouth neurocranial trabecula decreased size, abnormal
Protruding-mouth palatoquadrate arch absent, abnormal
Protruding-mouth palatoquadrate arch decreased size, abnormal
Protruding-mouth pharyngeal arch 3-7 decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.