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ZIRC
ZFIN ID: ZDB-FIG-150224-12
Kjaer-Sorensen et al., 2014 - Papp-a2 modulates development of cranial cartilage and angiogenesis in zebrafish embryos. Journal of Cell Science   127(23):5027-37 Full text @ J. Cell Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 1k-cell to Adult

Fig. 2

Expression analysis of papp-a2 in zebrafish embryos, larvae and adults. (A) Quantitative RT-PCR analysis of papp-a2 mRNA in adult tissues. Analysis was performed on pooled organs from three females, with the exception of testes, which were dissected and pooled from two males. ND, not detected. (B) Quantitative RT-PCR analysis of papp-a2 expression levels during early development (30–50 embryos per group). papp-a2 expression was normalized relative to that of beta-2-microglobulin and fold change was calculated relative to bone (A) or 96 hpf (B), respectively. Plotted values are mean±s.d. of triplicate measurements. (C–G) Whole mount in situ hybridization showing papp-a2 expression at 12 (C), 18 (D), 24 (E), and 48 hpf (F,G). G is a magnified section of a stained 48 hpf embryo showing expression in the lower jaw (*). Anterior to the left, dorsal to the top, except 12 hpf, which is dorsal view, animal pole to the top. cm, chordamesoderm; nc, notochord; mb, midbrain; hb, hindbrain; cb, cerebellum; rgc, retinal ganglion cell layer; arrowheads, hindbrain ganglia. Identical staining patterns were observed when using two other non-overlapping probes, and no signal was observed using sense probe at any developmental stage (not shown).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
pappa2 WT standard conditions 1k-cell to Day 4 whole organism RTPCR
5-9 somites axial mesoderm ISH
14-19 somites central nervous system anterior region ISH
14-19 somites notochord posterior side ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ISH
Prim-5 notochord posterior side ISH
Long-pec cerebellum ISH
Long-pec hindbrain ganglion ISH
Long-pec retinal ganglion cell layer ISH
Long-pec ventral mandibular arch ISH
Adult bone tissue RTPCR
Adult brain RTPCR
Adult eye RTPCR
Adult gill RTPCR
Adult gut RTPCR
Adult Not Detected heart RTPCR
Adult kidney RTPCR
Adult Not Detected liver RTPCR
Adult muscle RTPCR
Adult ovary RTPCR
Adult testis RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Journal of Cell Science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Cell Sci.