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ZFIN ID: ZDB-FIG-150115-35
Fukuhara et al., 2014 - Visualizing the cell-cycle progression of endothelial cells in zebrafish. Developmental Biology   393(1):10-23 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 4

Fig. 7

Cell-cycle progression of ECs during the late stage of vascular development. (A) 3D-rendered confocal images of the trunk (left) and caudal (right) vasculature in the Tg(flk1:mV-zGem);(fli1:Myr-mC) embryos at 2, 3 and 4 dpf as indicated at the left. Left, mVenus image; right, the merged image of mVenus (green) and mCherry (red). Scale bars, 100 μm. (B) The numbers of mVenus-positive ECs in the trunk and caudal vasculature at 2, 3 and 4 dpf, as observed in A, were counted and shown as meansĀ±s.d. (2 dpf [n=16], 3 dpf [n=14], 4 dpf [n=13]). (C) The numbers of mVenus-positive ECs in the DLAV, CA, arterial ISV (aISV), CV/CVP and venous ISV (vISV) of the caudal vasculature at 2 and 3 dpf were quantified, and then expressed as percentages of the total number (2 dpf [n=97], 3 dpf [n=28]).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mCherry ncv1Tg; ncv3Tg standard conditions Long-pec post-vent vasculature IFL
Long-pec trunk vasculature IFL
Protruding-mouth post-vent vasculature IFL
Protruding-mouth trunk vasculature IFL
Day 4 post-vent vasculature IFL
Day 4 trunk vasculature IFL
Venus ncv1Tg; ncv3Tg standard conditions Long-pec post-vent vasculature endothelial cell IFL
Long-pec trunk vasculature endothelial cell IFL
Protruding-mouth post-vent vasculature endothelial cell IFL
Protruding-mouth trunk vasculature endothelial cell IFL
Day 4 Not Detected posterior cardinal vein endothelial cell IFL
Day 4 Not Detected trunk vasculature endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 393(1), Fukuhara, S., Zhang, J., Yuge, S., Ando, K., Wakayama, Y., Sakaue-Sawano, A., Miyawaki, A., Mochizuki, N., Visualizing the cell-cycle progression of endothelial cells in zebrafish, 10-23, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.