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ZFIN ID: ZDB-FIG-150115-29
Fukuhara et al., 2014 - Visualizing the cell-cycle progression of endothelial cells in zebrafish. Developmental Biology   393(1):10-23 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 1

Development and characterization of transgenic zebrafish lines expressing zFucci cell-cycle biosensors in endothelial cells (ECs). (A) Schematic representation of the plasmids used for delivering transgenes expressing mVenus-zGem (1/100) (mV-zGem) and mCherry-zCdt1(1/190) (mC-zCdt) under control of the flk1 promoter. (B) Upper, fluorescence image (mV-zGem [green] and mC-zCdt1 [red]) of an EC-zFucci, Tg(flk1:mV-zGem);(flk1:mC-Cdt) embryo, at 31 hpf; lower, bright field images. (C) 3D-rendered confocal stack fluorescence images of the caudal regions of the Tg(flk1:mV-zGem);(fli1:Myr-mC) embryo at 33 hpf. Upper, mVenus image; lower, the merged image of mVenus (green) and mCherry (red). All of the confocal fluorescence images are lateral views and displayed as anterior to the left, unless otherwise described, in the following images. (D) 3D-rendered confocal images of the caudal regions of the Tg(flk1:mC-Cdt);(fli1:Myr-GFP) embryo at 48 hpf. Upper, mCherry image; lower, the merged image of mVenus (green) and mCherry (red). (E) Images of the EC-zFucci embryo at 29.5 hpf treated with EdU for 1 h. mVenus images (green), mCherry images (red) and EdU images visualized by Alexa 647-azide (blue) of the trunk vessel are shown as indicated at the lower left corner of the image. Yellow, pink and white arrowheads indicate mV-zGem/EdU double-positive cells, mV-zGem-positive/EdU-negative cells and mC-zCdt-positive/EdU-negative cells, respectively. (F) Percentages of mV-zGem-positive, mC-zCdt-positive and mV-zGem/mC-zCdt double positive cells labeled without () or with (+) EdU as observed in E were quantified (n=55). Scale bars, 200 μm (B) and 100 μm (C–E).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ncv2Tg; ncv4Tg standard conditions Long-pec trunk vasculature IFL
mCherry ncv1Tg; ncv3Tg standard conditions Prim-15 trunk vasculature IFL
ncv2Tg; ncv4Tg standard conditions Long-pec trunk vasculature endothelial cell IFL
ncv3Tg; ncv4Tg standard conditions Prim-5 trunk vasculature endothelial cell IFL
Prim-15 endothelial cell IFL
Venus ncv1Tg; ncv3Tg standard conditions Prim-15 trunk vasculature endothelial cell IFL
ncv3Tg; ncv4Tg standard conditions Prim-5 trunk vasculature endothelial cell IFL
Prim-15 endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 393(1), Fukuhara, S., Zhang, J., Yuge, S., Ando, K., Wakayama, Y., Sakaue-Sawano, A., Miyawaki, A., Mochizuki, N., Visualizing the cell-cycle progression of endothelial cells in zebrafish, 10-23, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.