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ZFIN ID: ZDB-FIG-150115-34
Fukuhara et al., 2014 - Visualizing the cell-cycle progression of endothelial cells in zebrafish. Developmental Biology   393(1):10-23 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 6

Cell-cycle progression of ECs during formation of hindbrain vasculature. (A) 3D-rendered confocal images of the hindbrain vasculature in Tg(flk1:mV-zGem);(fli1:Myr-mC) embryos at 28 hpf. Left, mVenus image; right, the merged image of mVenus (green) and mCherry. All confocal images in this figure are dorsal views and displayed as anterior to the top. (B, C) 3D-rendered confocal fluorescence image of the hindbrain vasculature in Tg(flk1:mV-zGem);(fli1:Myr-mC) embryos at 28.5 hpf (B) or 34 hpf (C) and the corresponding subsequent time-lapse images at the elapsed time (h:min) indicated at the bottom of the upper panel. Upper, mVenus image; lower, the merged image of mVenus (green) and mCherry. Arrowheads indicate mVenus-positive ECs that sprout from the primordial hindbrain channels (PHBCs) (B) and the ECs that caudally migrate to form the basilar artery (BA) (C). (D) Confocal fluorescence image of the hindbrain vasculature in 36 hpf Tg(fli1:Myr-mC);( flk1:NLS-Eos) embryos treated from 27 hpf with DMSO (left) or HU/APH (right). mCherry and the merged images of mCherry (red) and Eos (green) are shown as indicated at the top. Arrowheads indicate defective formation of the BA. (E) Confocal fluorescence images of the hindbrain vasculature in Tg(flk1:mV-zGem);(fli1:Myr-mC) embryos at 39 hpf and the corresponding subsequent time-lapse images at the elapsed time (h:min) indicated at the bottom of the upper panel are shown, as in B. Arrowheads indicate mVenus-positive ECs that sprout from PHBCs to form the CtAs. (F) Confocal fluorescence images of the hindbrain vasculature in 48 hpf Tg(fli1:Myr-mC);( flk1:NLS-Eos) embryos treated from 34 hpf with DMSO (left) or HU/APH (right) are shown, as in D. Asterisks and arrowheads indicate CtAs and ECs failing to sprout from PHBCs to form the CtAs, respectively. PHBC, primordial hindbrain channels; LDA, lateral dorsal aorta; BA, basilar artery; CtA, central artery. Scale bars, 100 μm (A–F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Eos ncv1Tg; ncv6Tg control Prim-25 basilar artery endothelial cell IFL
Prim-25 brain vasculature endothelial cell IFL
Prim-25 primordial hindbrain channel endothelial cell IFL
Long-pec basilar artery endothelial cell IFL
Long-pec brain vasculature endothelial cell IFL
Long-pec primordial hindbrain channel endothelial cell IFL
ncv1Tg; ncv6Tg chemical treatment: pharmaceutical Prim-25 basilar artery endothelial cell IFL
Prim-25 brain vasculature endothelial cell IFL
Prim-25 primordial hindbrain channel endothelial cell IFL
Long-pec basilar artery endothelial cell IFL
Long-pec brain vasculature endothelial cell IFL
Long-pec primordial hindbrain channel endothelial cell IFL
mCherry ncv1Tg; ncv3Tg standard conditions Prim-5 brain vasculature IFL
Prim-5 lateral dorsal aorta IFL
Prim-5 primordial hindbrain channel IFL
Prim-5 to Prim-15 brain vasculature IFL
Prim-5 to Prim-15 primordial hindbrain channel IFL
Prim-15 to High-pec basilar artery IFL
Prim-15 to High-pec brain vasculature IFL
Prim-15 to High-pec primordial hindbrain channel IFL
Prim-25 to High-pec basilar artery IFL
Prim-25 to High-pec brain vasculature IFL
Prim-25 to High-pec primordial hindbrain channel IFL
ncv1Tg; ncv6Tg control Prim-25 basilar artery IFL
Prim-25 brain vasculature IFL
Prim-25 primordial hindbrain channel IFL
Long-pec basilar artery IFL
Long-pec brain vasculature IFL
Long-pec primordial hindbrain channel IFL
ncv1Tg; ncv6Tg chemical treatment: pharmaceutical Prim-25 basilar artery IFL
Prim-25 brain vasculature IFL
Prim-25 primordial hindbrain channel IFL
Long-pec basilar artery IFL
Long-pec brain vasculature IFL
Long-pec primordial hindbrain channel IFL
Venus ncv1Tg; ncv3Tg standard conditions Prim-5 brain vasculature endothelial cell IFL
Prim-5 primordial hindbrain channel endothelial cell IFL
Prim-5 to Prim-15 brain vasculature endothelial cell IFL
Prim-5 to Prim-15 primordial hindbrain channel endothelial cell IFL
Prim-15 to High-pec brain vasculature endothelial cell IFL
Prim-15 to High-pec primordial hindbrain channel endothelial cell IFL
Prim-25 to High-pec brain vasculature endothelial cell IFL
Prim-25 to High-pec primordial hindbrain channel endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 393(1), Fukuhara, S., Zhang, J., Yuge, S., Ando, K., Wakayama, Y., Sakaue-Sawano, A., Miyawaki, A., Mochizuki, N., Visualizing the cell-cycle progression of endothelial cells in zebrafish, 10-23, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.