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ZFIN ID: ZDB-FIG-150115-30
Fukuhara et al., 2014 - Visualizing the cell-cycle progression of endothelial cells in zebrafish. Developmental Biology   393(1):10-23 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec

Fig. 2

Validation of the EC-zFucci Tg fish line. (A) Confocal 3D fluorescence image of the intersegmental vessel (ISV) of an EC-zFucci embryo at 36 hpf (first column) and the corresponding subsequent time-lapse images (from second to sixth columns) at the elapsed time (h:min) indicated at the bottom. Top, mVenus images; middle, mCherry images; bottom, the merged images. Note that an mV-zGem-positive cell (arrows at 0:00 and 0:20) divides into two daughter cells (arrows at 0:50), which subsequently lose mVenus fluorescence and start to emit mCherry fluorescence (arrows at 3:10, 3:50 and 8:10). (B) Confocal 3D fluorescence image of the ISV of an EC-zFucci embryo at 38 hpf (first column) and the corresponding subsequent time-lapse images (from second to seventh columns) at the elapsed time (h:min) indicated at the bottom are shown, essentially as in A. Note that the mC-zCdt-positive cell (arrows at 0:00 and 1:00) loses mCherry fluorescence (arrow at 1:10), starts to emit mVenus fluorescence (arrows at 2:30, 5:00 and 6:00), and subsequently divides into two daughter cells (arrows at 6:20 and 7:00). (C) 3D-rendered confocal images of the caudal regions of 48 hpf Tg(flk1:mV-zGem);(fli1:Myr-mC) embryos treated with either DMSO or both hydroxyurea and aphidicolin (HU/APH) from 30 to 48 hpf. Upper, mVenus images; lower, the merged images of mVenus and mCherry. Scale bars, 100 μm. (D) Duration: time that mVenus-positive cells in the EC-zFucci Tg embryos at 36 hpf continue to emit mVenus fluorescence. Tg(flk1:mV-zGem);(fli1:Myr-mC) embryos were treated from 30 hpf with either DMSO (closed circles) or HU/APH (open circles), and time-lapse imaged starting at 36 hpf for 9 h 20 min to track the mVenus-positive cells. The y-axis indicates the number of mVenus-positive cells, while the x-axis shows the time after starting the time-lapse imaging. Data are expressed as percentages relative to that observed at the beginning of the imaging (DMSO [n=35], HU/APH [n=41]).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry ncv1Tg; ncv3Tg control Long-pec trunk vasculature IFL
ncv1Tg; ncv3Tg chemical treatment: pharmaceutical Long-pec trunk vasculature IFL
ncv3Tg; ncv4Tg standard conditions Prim-25 intersegmental vessel endothelial cell IFL
Venus ncv1Tg; ncv3Tg control Long-pec trunk vasculature endothelial cell IFL
ncv1Tg; ncv3Tg chemical treatment: pharmaceutical Long-pec trunk vasculature endothelial cell IFL
ncv3Tg; ncv4Tg standard conditions Prim-25 intersegmental vessel endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 393(1), Fukuhara, S., Zhang, J., Yuge, S., Ando, K., Wakayama, Y., Sakaue-Sawano, A., Miyawaki, A., Mochizuki, N., Visualizing the cell-cycle progression of endothelial cells in zebrafish, 10-23, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.