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ZFIN ID: ZDB-FIG-150115-32
Fukuhara et al., 2014 - Visualizing the cell-cycle progression of endothelial cells in zebrafish. Developmental Biology   393(1):10-23 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 26+ somites to Long-pec

Fig. 4

Cell-cycle progression of ECs during caudal vessel formation. (A) 3D-rendered confocal images of the caudal vessels of Tg(flk1:mV-zGem);(fli1:Myr-mC) embryos at 24, 30, 36 and 48 hpf as indicated in the first column from the left. Bright field images (left) and fluorescence images (as indicated at the top). The boxed areas of the second and the third columns are enlarged in the fourth and fifth columns, respectively. CA, caudal vessel; VC, vascular cord; ISV, intersegmental vessel; CVP, caudal vein plexus; DLAV, dorsal longitudinal anastomotic vessels. Scale bars, 100 μm. (B) Time-lapse confocal imaging of caudal vessel formation in the early stage (from 25 to 29 hpf) in the Tg(flk1:mV-zGem);(flk1:NLS-mC) embryos. The confocal 3D image of the embryo at 25 hpf (top) and subsequent time-lapse images (from second to fourth rows) as indicated by the elapsed time (hr:min) at the top right. Left, merged (mVenus and mCherry); center, enlarged mVenus images of the boxed region of the left panel; right, enlarged merged image of the boxed region of the left panel. The cell movement of the posterior-most cell at the beginning (crosses) and at the end (circles) during time-lapse imaging, as analyzed employing a cell tracking application. (C) Tg(flk1:mV-zGem);(flk1:NLS-mC) embryos in the late stage (from 33–37 hpf) were similarly time-lapse imaged and analyzed as in B. Note that NLS-mCherry-positive cells merge just posterior to the growing edges of caudal vessels in the early stage, but not in the late stage, of caudal vessel formation. (D) Time-lapse confocal imaging of caudal vessel formation at the early stage (from 23 to 27 hpf) in the Tg(flk1:NLS-Eos) embryos. Confocal 3D images (at 23 hpf) and subsequent time-lapse images of photoconverted NLS-Eos (red)-marked ECs (encircled) from original NLS-Eos (green)-marked cells by laser irradiation at 23 hpf. Elapsed time (hr:min). Upper, merged images of Eos (green) and photoconverted Eos (red); lower, photoconverted Eos (red) images. Arrowheads indicate the anteriormost and posteriormost of the photoconverted cells. (E) Tg(flk1:NLS-Eos) embryos in the late stage (from 33 to 40 hpf) were time-lapse imaged and analyzed as in D.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Eos ncv6Tg standard conditions 26+ somites to Prim-5 trunk vasculature endothelial cell IFL
Prim-15 to Prim-25 trunk vasculature endothelial cell IFL
mCherry ncv1Tg; ncv3Tg standard conditions Prim-5 caudal artery IFL
Prim-5 trunk vasculature IFL
Prim-15 caudal artery IFL
Prim-15 caudal vein plexus IFL
Prim-15 dorsal aorta IFL
Prim-15 intersegmental vessel IFL
Prim-15 posterior cardinal vein IFL
Prim-15 trunk vasculature IFL
Prim-25 caudal artery IFL
Prim-25 caudal vein plexus IFL
Prim-25 dorsal aorta IFL
Prim-25 dorsal longitudinal anastomotic vessel IFL
Prim-25 intersegmental vessel IFL
Prim-25 posterior cardinal vein IFL
Prim-25 trunk vasculature IFL
Long-pec caudal artery IFL
Long-pec caudal vein plexus IFL
Long-pec dorsal aorta IFL
Long-pec dorsal longitudinal anastomotic vessel IFL
Long-pec intersegmental vessel IFL
Long-pec posterior cardinal vein IFL
Long-pec trunk vasculature IFL
ncv3Tg; ncv5Tg standard conditions Prim-5 trunk vasculature endothelial cell IFL
Prim-15 trunk vasculature endothelial cell IFL
Venus ncv1Tg; ncv3Tg standard conditions Prim-5 trunk vasculature endothelial cell IFL
Prim-15 caudal artery endothelial cell IFL
Prim-15 intersegmental vessel endothelial cell IFL
Prim-15 trunk vasculature endothelial cell IFL
Prim-25 caudal artery endothelial cell IFL
Prim-25 intersegmental vessel endothelial cell IFL
Prim-25 trunk vasculature endothelial cell IFL
Long-pec caudal vein plexus endothelial cell IFL
Long-pec intersegmental vessel endothelial cell IFL
Long-pec trunk vasculature endothelial cell IFL
ncv3Tg; ncv5Tg standard conditions Prim-5 trunk vasculature endothelial cell IFL
Prim-15 trunk vasculature endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 393(1), Fukuhara, S., Zhang, J., Yuge, S., Ando, K., Wakayama, Y., Sakaue-Sawano, A., Miyawaki, A., Mochizuki, N., Visualizing the cell-cycle progression of endothelial cells in zebrafish, 10-23, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.