ZFIN ID: ZDB-FIG-130826-57
Pascoal et al., 2013 - Notch signalling is required for the formation of structurally stable muscle fibres in zebrafish. PLoS One   8(6):e68021 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 4

Notch signalling is crucial for skeletal muscle integrity and stress fibres formation.

Immunostaining of 5 dpf pectoral fins (A–G′′) with DAPI and phalloidin to label nuclei (blue) and filamentous actin (green), respectively. Stress fibres in the endoskeletal disc cells and intact skeletal muscle fibres are formed in the pectoral fins of siblings (n = 20) (A–A′′) and in DMSO-treated embryos (n = 17) (F-F′′). In the pectoral fins were Notch signalling was disrupted like in mibta52b (n = 22) (B–B′′) and mibm178 (n = 10) (C–C′′) mutants, jagged2 (n = 11) (D–D′′) and Su(H)1+2 (n = 10) (E–E′′) morphants and in DAPT-treated embryos (n = 16) (G–G′′) the endoskeletal disc cells present high levels of actin at the periphery and the skeletal muscle fibres are wavy with gaps between them.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-jag2b standard conditions Day 5 pectoral fin actin filament mislocalised, abnormal
Day 5 pectoral fin musculature contractile fiber malformed, abnormal
WT + MO5-rbpja,rbpjb standard conditions Day 5 pectoral fin actin filament mislocalised, abnormal
Day 5 pectoral fin musculature contractile fiber malformed, abnormal
mib1m178/m178 standard conditions Day 5 pectoral fin actin filament mislocalised, abnormal
Day 5 pectoral fin musculature contractile fiber malformed, abnormal
mib1ta52b/ta52b standard conditions Day 5 pectoral fin actin filament mislocalised, abnormal
Day 5 pectoral fin musculature contractile fiber malformed, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One