ZFIN ID: ZDB-FIG-130826-54
Pascoal et al., 2013 - Notch signalling is required for the formation of structurally stable muscle fibres in zebrafish. PLoS One   8(6):e68021 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec

Fig. 1

Expression pattern of Notch signalling pathway genes during pectoral fin development.

Whole-mount in situ hybridization of 48 hpf embryos shows strong expression of jagged2 (n = 20) (A), notch1a (n = 22) (B), notch2 (n = 22) (C), notch3 (n = 20) (D), her6 (n = 25) (E), her7 (n = 23) (F), her13.2 (n = 23) (G), mib1 (n = 22) (H) and mib2 (n = 25) (I) in the pectoral fins. At this time point it is possible to detect expression of jagged2 (A′, A′′) and notch3 (D′, D′′) lining the base of the apical ectodermal fold and in the myogenic mesenchyme, notch1a (B′, B′′), her6 (L′, L′′), her13.2 (N′, N′′), mib1 (H′, H′′) and mib2 (I′, I′′) in the myogenic mesenchyme and notch2 (J′, J′′) and her7 (M′, M′′) in the entire fin. (A′–I′) Detached pectoral fins with distal to the top. (A′′-I′′) Transversal sections at the level of pectoral fins. AEF, apical ectodermal fold; dmm, dorsal myogenic mesenchyme; vmm, ventral myogenic mesenchyme; cc, chondrogenic condensation.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
her6 WT standard conditions Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ISH
her7 WT standard conditions Long-pec pectoral fin ISH
hes6 WT standard conditions Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ISH
jag2b WT standard conditions Long-pec apical ectodermal ridge ISH
Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ISH
mib1 WT standard conditions Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ISH
mib2 WT standard conditions Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ISH
notch1a WT standard conditions Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ISH
notch2 WT standard conditions Long-pec pectoral fin ISH
notch3 WT standard conditions Long-pec apical ectodermal ridge ISH
Long-pec mesenchyme pectoral fin ISH
Long-pec pectoral fin ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One