ZFIN ID: ZDB-FIG-130802-1
Shin et al., 2012 - Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver. Mechanisms of Development   128(11-12):525-535 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Day 4

Fig. 1

Liver formation and the expression patterns of two hepatoblast markers, hhex and prox1, in wnt2bb mutants. (A-D) The Tg(fabp10:dsRed,ela3l:EGFP);Tg(ins:dsRed) line was used to reveal the liver (red), acinar pancreas (green), and pancreatic beta cells (red). Arrows point to the liver, arrowheads to the endocrine pancreas. The liver formed in most wnt2bb mutants (B and C), but its size was variable and smaller than wild-type. Dorsal views, anterior up. (E-T) hhex expression in the liver-forming region was weakly reduced in wnt2bb mutants compared to wild-type at 30 and 36 hpf (F and H, arrows), whereas prox1 expression was greatly reduced (N and P, arrows). hhex expression in the dorsal pancreas (E-H, arrowheads) and prox1 expression in the interrenal primordium (M-P, arrowheads) appeared unaffected in wnt2bb mutants compared to wild-type. To better visualize hepatic prox1 expression, side-view images are shown in an inset (M-P). Both hhex and prox1 are expressed in the developing liver, but only hhex is expressed in the extrahepatic duct (I-L, arrows). This extrahepatic duct expression appears unaffected in wnt2bb mutants (J and L, arrows). Dorsal views, anterior to the left. Scale bars, 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed gz15Tg; m1018Tg standard conditions Day 4 liver IFL
Day 4 pancreatic B cell IFL
wnt2bbs403/s403; gz15Tg; m1018Tg standard conditions Day 4 liver IFL
Day 4 pancreatic B cell IFL
EGFP gz15Tg; m1018Tg standard conditions Day 4 exocrine pancreas IFL
wnt2bbs403/s403; gz15Tg; m1018Tg standard conditions Day 4 exocrine pancreas IFL
hhex WT standard conditions Prim-15 hepatoblast ISH
Prim-15 posterior pancreatic bud ISH
Prim-25 hepatoblast ISH
Prim-25 posterior pancreatic bud ISH
Long-pec hepatoblast ISH
Long-pec posterior pancreatic bud ISH
Pec-fin endocrine pancreas ISH
Pec-fin hepatic duct ISH
Pec-fin hepatoblast ISH
wnt2bbs403/s403 standard conditions Prim-15 hepatoblast ISH
Prim-15 posterior pancreatic bud ISH
Prim-25 hepatoblast ISH
Prim-25 posterior pancreatic bud ISH
Long-pec hepatoblast ISH
Long-pec posterior pancreatic bud ISH
Pec-fin endocrine pancreas ISH
Pec-fin hepatic duct ISH
Pec-fin hepatoblast ISH
prox1a WT standard conditions Prim-15 hepatoblast ISH
Prim-15 interrenal primordium ISH
Prim-25 hepatoblast ISH
Prim-25 interrenal primordium ISH
Long-pec hepatoblast ISH
Long-pec interrenal primordium ISH
Pec-fin hepatoblast ISH
Pec-fin interrenal primordium ISH
wnt2bbs403/s403 standard conditions Prim-15 hepatoblast ISH
Prim-15 interrenal primordium ISH
Prim-25 hepatoblast ISH
Prim-25 interrenal primordium ISH
Long-pec hepatoblast ISH
Long-pec interrenal primordium ISH
Pec-fin hepatoblast ISH
Pec-fin interrenal primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
wnt2bbs403/s403 standard conditions Prim-15 liver absent, abnormal
Prim-15 liver decreased size, abnormal
Prim-15 liver development disrupted, abnormal
Prim-15 liver increased variability of size, abnormal
Prim-25 liver absent, abnormal
Prim-25 liver decreased size, abnormal
Prim-25 liver development disrupted, abnormal
Prim-25 liver increased variability of size, abnormal
Long-pec liver absent, abnormal
Long-pec liver decreased size, abnormal
Long-pec liver development disrupted, abnormal
Long-pec liver increased variability of size, abnormal
Pec-fin liver absent, abnormal
Pec-fin liver decreased size, abnormal
Pec-fin liver development disrupted, abnormal
Pec-fin liver increased variability of size, abnormal
wnt2bbs403/s403; gz15Tg; m1018Tg standard conditions Day 4 liver absent, abnormal
Day 4 liver decreased size, abnormal
Day 4 liver development disrupted, abnormal
Day 4 liver increased variability of size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 128(11-12), Shin, D., Weidinger, G., Moon, R.T., and Stainier, D.Y., Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver, 525-535, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.