ZFIN ID: ZDB-FIG-130807-28
Shin et al., 2012 - Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver. Mechanisms of Development   128(11-12):525-535 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Pec-fin
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Pec-fin

Fig. 6

Both the liver and the extrahepatic duct form in Wnt/β-catenin signaling deficient embryo slacking Fgf10a function. Tg(hs:dkk1-GFP);wnt2bb-/- embryos were injected with fgf10a MO, heat-shocked at 18 hpf, and examined at 54 (E) or 70 (D) hpf. The extrahepatic duct was revealed by mAb 2F11 staining, the exocrine pancreas by Tg(ptf1a:GFP) expression, and the liver by Tg(fabp10:RFP) (A-D) or Prox1 (E) expression. Both the liver (arrows) and the extrahepatic duct (ehd) failed to form in Wnt/β-catenin signaling deficient embryos (C), whereas they did form when these embryos were injected with fgf10a MO (D and E). The gallbladder (arrowheads) formed in the extrahepatic duct when the liver formed, whereas it formed in the extrapancreatic duct (epd) when the liver failed to form (C). (A′-E′) The liver, the extrahepatic and extrapancreatic ducts as well as the pancreas are schematically illustrated. Ventral views, anterior up. Scale bar, 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed gz15Tg; jh1Tg standard conditions Pec-fin liver IFL
wnt2bbs403/s403; gz15Tg; jh1Tg standard conditions Pec-fin liver IFL
wnt2bbs403/s403; gz15Tg; jh1Tg; w32Tg heat shock Pec-fin Not Detected liver IFL
wnt2bbs403/s403; gz15Tg; jh1Tg; w32Tg + MO4-fgf10a heat shock Pec-fin liver IFL
EGFP gz15Tg; jh1Tg standard conditions Pec-fin pancreas IFL
wnt2bbs403/s403; gz15Tg; jh1Tg standard conditions Pec-fin pancreas IFL
wnt2bbs403/s403; gz15Tg; jh1Tg; w32Tg heat shock Pec-fin pancreas IFL
wnt2bbs403/s403; gz15Tg; jh1Tg; w32Tg + MO4-fgf10a heat shock Long-pec pancreas IFL
Pec-fin pancreas IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-2F11 IHC gz15Tg; jh1Tg standard conditions Pec-fin extrapancreatic duct
IHC Pec-fin gall bladder
IHC Pec-fin hepatic duct
IHC wnt2bbs403/s403; gz15Tg; jh1Tg standard conditions Pec-fin extrapancreatic duct
IHC Pec-fin gall bladder
IHC Pec-fin hepatic duct
IHC wnt2bbs403/s403; gz15Tg; jh1Tg; w32Tg heat shock Pec-fin extrapancreatic duct
IHC Pec-fin gall bladder
IHC Pec-fin Not Detected hepatic duct
IHC wnt2bbs403/s403; gz15Tg; jh1Tg; w32Tg + MO4-fgf10a heat shock Long-pec extrapancreatic duct
IHC Long-pec hepatic duct
IHC Pec-fin extrapancreatic duct
IHC Pec-fin gall bladder
IHC Pec-fin hepatic duct
Ab3-prox1 IHC wnt2bbs403/s403; gz15Tg; jh1Tg; w32Tg + MO4-fgf10a heat shock Long-pec liver
Phenotype Details
Fish Conditions Stage Phenotype
wnt2bbs403/s403; gz15Tg; jh1Tg standard conditions Pec-fin extrahepatic duct morphology, abnormal
Pec-fin extrahepatic duct present, normal
Pec-fin gall bladder development disrupted, abnormal
Pec-fin gall bladder morphology, abnormal
wnt2bbs403/s403; gz15Tg; jh1Tg; w32Tg standard conditions Pec-fin extrahepatic duct absent, abnormal
Pec-fin extrapancreatic duct present, normal
Pec-fin gall bladder development disrupted, abnormal
Pec-fin gall bladder morphology, abnormal
Pec-fin gall bladder position, abnormal
wnt2bbs403/s403; gz15Tg; jh1Tg; w32Tg + MO4-fgf10a heat shock Long-pec extrahepatic duct morphology, abnormal
Long-pec extrapancreatic duct present, normal
Long-pec gall bladder absent, abnormal
Long-pec gall bladder development disrupted, abnormal
Pec-fin extrahepatic duct morphology, abnormal
Pec-fin extrapancreatic duct present, normal
Pec-fin gall bladder development disrupted, abnormal
Pec-fin gall bladder morphology, abnormal
Pec-fin gall bladder position, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 128(11-12), Shin, D., Weidinger, G., Moon, R.T., and Stainier, D.Y., Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver, 525-535, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.