ZFIN ID: ZDB-FIG-130807-1
Shin et al., 2012 - Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver. Mechanisms of Development   128(11-12):525-535 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 4

Sox32 positively regulates liver formation in wnt2bb mutants. (A) Schematic of the dominant-negative Sox32 construct. The transcriptional repressor domain of Drosophila Engrailed was fused to the HMG box (gray box) of Sox32. (B-E) mRNA injections into Tg(sox17:GFP) embryos reveal that dnSox32 blocks Sox32 function. Injections of sox32 mRNA enhanced GFP expression driven by the sox17 promoter; injections of dnsox32 mRNA reduced it. Co-injections of sox32 and dnsox32 mRNA blocked GFP induction by Sox32. Dorsal views, anterior up. (F-H) Hepatic sox17 expression was almost absent (G, 60%) or greatly reduced (H, 40%) in Tg(hs:dnsox32) embryos heat-shocked at 26 hpf (n = 10). Dorsal views, anterior to the left. (I-L) Tg(hs:dnsox32);wnt2bb-/- embryos were heat-shocked at 26 hpf, and examined at 5.5 dpf. The liver (arrows) formed in all Tg(hs:dnsox32), but failed to form in most Tg(hs:dnsox32);wnt2bb-/- embryos (L, 22 out of 25). Side views, anterior up. Scale bars, 100 ┬Ám.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed gz15Tg standard conditions Day 5 liver IFL
gz15Tg; s921Tg heat shock Day 5 liver IFL
wnt2bbs403/s403; gz15Tg standard conditions Day 5 liver IFL
wnt2bbs403/s403; gz15Tg; s921Tg heat shock Day 5 Not Detected liver IFL
EGFP gz15Tg standard conditions Day 5 exocrine pancreas IFL
gz15Tg; s921Tg heat shock Day 5 exocrine pancreas IFL
wnt2bbs403/s403; gz15Tg standard conditions Day 5 exocrine pancreas IFL
wnt2bbs403/s403; gz15Tg; s921Tg heat shock Day 5 exocrine pancreas IFL
sox17 WT standard conditions Long-pec liver ISH
s921Tg heat shock Long-pec liver ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz15Tg; s921Tg heat shock Day 5 liver present, normal
Day 5 pancreas present, normal
s921Tg heat shock Long-pec liver absent, abnormal
Long-pec liver decreased size, abnormal
Long-pec liver development disrupted, abnormal
wnt2bbs403/s403; gz15Tg standard conditions Day 5 liver present, normal
Day 5 pancreas present, normal
wnt2bbs403/s403; gz15Tg; s921Tg heat shock Day 5 liver absent, abnormal
Day 5 liver development disrupted, abnormal
Day 5 pancreas present, normal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 128(11-12), Shin, D., Weidinger, G., Moon, R.T., and Stainier, D.Y., Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver, 525-535, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.