ZFIN ID: ZDB-FIG-130807-2
Shin et al., 2012 - Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver. Mechanisms of Development   128(11-12):525-535 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Day 4 to Day 5
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Day 4 to Day 5

Fig. 5

fgf10a signaling negatively regulates liver formation in wnt2bb mutants. (A) Wnt/β-catenin signaling deficient embryos were treated with SU5402 (1 µM) from 26 hpf onward, and examined at 5 dpf. The liver (arrow) formed in SU5402-treated embryos despite severe morphological defects in the head and trunk. Arrowheads point to the endocrine pancreas. (B) The liver (arrow) also formed in fgf10a-/-; Wnt/β-catenin signaling deficient embryos. Dorsal views, anterior up. Scale bars, 100 µm. (C) The time-window critical for Fgf function in liver formation was delineated by treating Wnt/β-catenin signaling deficient embryos with SU5402 at multiple time points and for different duration (black bars); recovery indicates the percentage of embryos showing a liver at 5 dpf (n = 9-23 for each treatment).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed fgf10atbvbo/tbvbo; wnt2bbs403/s403; gz15Tg; w32Tg heat shock Day 4 liver ISH
wnt2bbs403/s403; gz15Tg; m1018Tg; w32Tg heat shock Day 5 Not Detected liver IFL
wnt2bbs403/s403; gz15Tg; m1018Tg; w32Tg heat shock, chemical treatment: SU5402 Day 5 liver IFL
wnt2bbs403/s403; gz15Tg; w32Tg heat shock Day 4 Not Detected liver ISH
EGFP fgf10atbvbo/tbvbo; wnt2bbs403/s403; gz15Tg; w32Tg heat shock Day 4 exocrine pancreas ISH
wnt2bbs403/s403; gz15Tg; m1018Tg; w32Tg heat shock Day 5 exocrine pancreas IFL
wnt2bbs403/s403; gz15Tg; m1018Tg; w32Tg heat shock, chemical treatment: SU5402 Day 5 exocrine pancreas IFL
wnt2bbs403/s403; gz15Tg; w32Tg heat shock Day 4 exocrine pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fgf10atbvbo/tbvbo; wnt2bbs403/s403; gz15Tg; w32Tg heat shock Day 4 liver present, normal
wnt2bbs403/s403; gz15Tg; m1018Tg; w32Tg heat shock Day 5 liver absent, abnormal
Day 5 liver development disrupted, abnormal
Day 5 pancreas morphology, normal
Day 5 pancreas present, normal
wnt2bbs403/s403; gz15Tg; m1018Tg; w32Tg heat shock, chemical treatment: SU5402 Day 5 head morphology, abnormal
Day 5 liver present, normal
Day 5 pancreas present, normal
Day 5 trunk morphology, abnormal
wnt2bbs403/s403; gz15Tg; w32Tg heat shock Day 4 liver absent, abnormal
Day 4 liver development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 128(11-12), Shin, D., Weidinger, G., Moon, R.T., and Stainier, D.Y., Intrinsic and extrinsic modifiers of the regulative capacity of the developing liver, 525-535, Copyright (2012) with permission from Elsevier. Full text @ Mech. Dev.