ZFIN ID: ZDB-FIG-130625-39
Schmid et al., 2013 - Loss of ALS-associated TDP-43 in zebrafish causes muscle degeneration, vascular dysfunction, and reduced motor neuron axon outgrowth. Proc. Natl. Acad. Sci. USA   110(13):4986-91 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 1 Overview of human TDP-43 and zebrafish orthologs and Western blot of their loss-of-function alleles. (A) Schematic representation of human TDP-43 (turquois), zebrafish Tardbp (red), and zebrafish Tardbpl protein (orange); blue bars represent nuclear localization sequence; green bars represent nuclear export sequence; RRM, RNA recognition motif. (B, Top) Tardbp specific monoclonal antibody 4A12-111 detects the 43-kDa Tardbp protein in adult brain from wild-type and tardbplmde222-/- but not from tardbpmde198-/- and tardbpmde897-/- fish. Asterisk marks an unspecific band. (Middle) Tardbpl-specific monoclonal antibody 5F5-11 detects the approximately 34-kDa Tardbpl protein in adult brain from wild-type, tardbpmde198-/- and tardbpmde897-/- but not from tardbplmde222-/- fish. (Bottom) α-Tubulin serves as a loading control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
tardbp Ab5-tardbp AB standard conditions Adult brain WB
Ab5-tardbp tardbpmde198/mde198(AB) standard conditions Adult Not Detected brain WB
Ab5-tardbp tardbpmde897/mde897(AB) standard conditions Adult Not Detected brain WB
Ab5-tardbp tardbplmde222/mde222(AB) standard conditions Adult brain WB
tardbpl Ab1-tardbpl AB standard conditions Adult brain WB
Ab1-tardbpl tardbpmde198/mde198(AB) standard conditions Adult brain WB
Ab1-tardbpl tardbpmde897/mde897(AB) standard conditions Adult brain WB
Ab1-tardbpl tardbplmde222/mde222(AB) standard conditions Adult Not Detected brain WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tardbpmde198/mde198(AB) standard conditions Adult brain tardbp expression absent, abnormal
tardbpmde897/mde897(AB) standard conditions Adult brain tardbp expression absent, abnormal
tardbplmde222/mde222(AB) standard conditions Adult brain tardbpl expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proc. Natl. Acad. Sci. USA for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA