ZFIN ID: ZDB-FIG-130109-17
Clément et al., 2012 - Functional redundancy between Cdc14 phosphatases in zebrafish ciliogenesis. Developmental dynamics : an official publication of the American Association of Anatomists   241(12):1911-1921 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 20-25 somites to Long-pec

Fig. 3 Effect of Cdc14A1 loss of function in LR asymmetry establishment. ISH and expression pattern quantification in non-injected, cdc14a1 and cdc14b MO- injected embryos. A-D: nkx2.5 expression at 31 hpf. E–H: foxa3 expression at 50 hpf. I-L: spaw expression at 19 hpf. Scale bar = 100 μm. M: Rescue of LR asymmetry defect in Cdc14A1-depleted embryos. Heart tube shifting was determined at 31 hpf. Numbers of embryos analyzed are indicated. Data are represented as mean ± SEM. Statistics were done using Student′s t-Test (D,H,L) and are only presented for the left-sided heart tube class in M.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxa3 AB standard conditions Long-pec gut ISH
Long-pec liver ISH
Long-pec pancreas ISH
AB + MO1-cdc14aa standard conditions Long-pec gut ISH
Long-pec liver ISH
Long-pec pancreas ISH
AB + MO1-cdc14b standard conditions Long-pec gut ISH
Long-pec liver ISH
Long-pec pancreas ISH
nkx2.5 AB standard conditions Prim-15 heart tube ISH
AB + MO1-cdc14aa standard conditions Prim-15 heart tube ISH
AB + MO1-cdc14b standard conditions Prim-15 heart tube ISH
spaw AB standard conditions 20-25 somites lateral plate mesoderm left side ISH
AB + MO1-cdc14aa standard conditions 20-25 somites Not Detected lateral plate mesoderm ISH
AB + MO1-cdc14b standard conditions 20-25 somites lateral plate mesoderm right side ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-cdc14aa standard conditions 20-25 somites left/right pattern formation occurrence, abnormal
Prim-15 embryonic heart tube left/right pattern formation disrupted, abnormal
Prim-15 heart jogging disrupted, abnormal
Prim-15 left/right pattern formation occurrence, abnormal
Long-pec left/right pattern formation occurrence, abnormal
AB + MO1-cdc14b standard conditions 20-25 somites left/right pattern formation occurrence, abnormal
Prim-15 embryonic heart tube left/right pattern formation disrupted, abnormal
Prim-15 heart jogging disrupted, abnormal
Prim-15 left/right pattern formation occurrence, abnormal
Long-pec left/right pattern formation occurrence, abnormal
AB + MO2-cdc14aa standard conditions Prim-15 embryonic heart tube left/right pattern formation disrupted, abnormal
Prim-15 heart jogging disrupted, abnormal
Prim-15 left/right pattern formation occurrence, abnormal
Long-pec left/right pattern formation occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.