ZFIN ID: ZDB-FIG-120905-27
Filippi et al., 2012 - Analysis of transcriptional codes for zebrafish dopaminergic neurons reveals essential functions of Arx and Isl1 in prethalamic dopaminergic neuron development. Developmental Biology   369(1):133-149 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 5

arx is required for the formation of DA neurons in the prethalamus and in the preoptic area. Morpholino-injected embryos were analyzed by WISH to detect th (A-F, 96 hpf), slc6a3/dat (M-P, 72 hpf) or ddc (Q-T, 72 hpf) expression, as well as by anti-TH immunofluorescence (G-L, 72 hpf). Arx knockdown by Morpholino injection results in a reduction in the number of prethalamic group 1 DA neurons (arrows in B, E, H) and of the preoptic group (K, arrowhead points to the postoptic commissure). This phenotype is not caused by Morpholino off-target p53-mediated cell death, as we obtain the same result when co-injecting p53-MO as a control (C, F, I, L). Moreover, the prethalamic expression domains of slc6a3/dat (M–P) and ddc (Q–T) are also reduced in morphant embryos when compared to controls (arrows point to the prethalamus). U: Evaluation of the effect of Arx knockdown on th expression for each DA cluster. Both ATG (left) and splice (right) targeting morpholinos affected DC1 and PO groups development, also when co-injected with the p53-MO (+). The groups of the arx morphant larvae were compared with those of control MO injected larvae, which all developed normally and are summarized here in the left-most lanes (ctr). A–C,M,N,Q,R are lateral views; D–L, O, P, S, T are dorsal views; anterior is to the left. Scale bars: 100 μm. Abbreviations: AC, amacrine cells; DC1-6, diencephalic groups 1–6; LC, locus coeruleus; MO, medulla oblongata; OB, olfactory bulb; PO, preoptic area; poc, postoptic commissure; Pr, pretectum; SP, subpallium; TC, telencephalic cluster; vDC, ventral diencephalic cluster.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ddc WT standard conditions Protruding-mouth dopaminergic neuron ISH
Protruding-mouth preoptic area dopaminergic neuron ISH
Protruding-mouth ventral thalamus dopaminergic neuron ISH
WT + MO2-arxa standard conditions Protruding-mouth dopaminergic neuron ISH
Protruding-mouth preoptic area dopaminergic neuron ISH
Protruding-mouth ventral thalamus dopaminergic neuron ISH
slc6a3 WT standard conditions Protruding-mouth dopaminergic neuron ISH
Protruding-mouth preoptic area dopaminergic neuron ISH
Protruding-mouth ventral thalamus dopaminergic neuron ISH
WT + MO2-arxa standard conditions Protruding-mouth dopaminergic neuron ISH
Protruding-mouth preoptic area dopaminergic neuron ISH
Protruding-mouth ventral thalamus dopaminergic neuron ISH
th WT standard conditions Day 4 dopaminergic neuron ISH
Day 4 preoptic area dopaminergic neuron ISH
Day 4 ventral thalamus dopaminergic neuron ISH
WT + MO2-arxa standard conditions Day 4 dopaminergic neuron ISH
Day 4 preoptic area dopaminergic neuron ISH
Day 4 ventral thalamus dopaminergic neuron ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab6-th IHC WT standard conditions Protruding-mouth dopaminergic neuron
IHC Protruding-mouth preoptic area dopaminergic neuron
IHC Protruding-mouth ventral thalamus dopaminergic neuron
IHC WT + MO2-arxa standard conditions Protruding-mouth dopaminergic neuron
IHC Protruding-mouth preoptic area dopaminergic neuron
IHC Protruding-mouth ventral thalamus dopaminergic neuron
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-arxa standard conditions Protruding-mouth preoptic area dopaminergic neuron decreased amount, abnormal
Protruding-mouth ventral thalamus dopaminergic neuron decreased amount, abnormal
Day 4 preoptic area dopaminergic neuron decreased amount, abnormal
Day 4 ventral thalamus dopaminergic neuron decreased amount, abnormal
WT + MO2-arxa standard conditions Protruding-mouth preoptic area dopaminergic neuron decreased amount, abnormal
Protruding-mouth ventral thalamus dopaminergic neuron decreased amount, abnormal
Day 4 preoptic area dopaminergic neuron decreased amount, abnormal
Day 4 ventral thalamus dopaminergic neuron decreased amount, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 369(1), Filippi, A., Jainok, C., and Driever, W., Analysis of transcriptional codes for zebrafish dopaminergic neurons reveals essential functions of Arx and Isl1 in prethalamic dopaminergic neuron development, 133-149, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.