ZFIN ID: ZDB-FIG-120525-29
Gao et al., 2012 - Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish. PLoS One   7(5):e36516 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish: b1204Tg + MO1-dcc, b1204Tg + MO1-ntn1b, b1204Tg + MO2-neo1a, b1204Tg + MO4-ntn1a, b1204Tg + MO1-dcc + MO2-neo1a (all 7) expand
Knockdown Reagents: MO1-dcc, MO1-ntn1b, MO2-neo1a, MO4-ntn1a
Observed In: dorso-rostral cluster axon extension, dorso-rostral cluster neuron projection
Stage: Prim-25

Fig. 5

Effects of inhibition of Netrin1 or Neogenin1 function on the ADt axons.

(A) ADt neurons project axons dorsally when Netrin1 function is inhibited (ntn-MO). Knockdown of Neogenin1 function doesn’t cause ADt neuron to project axon dorsally (neo-MO). Images were processed as in Fig. 3B. The pixel intensity value of aberrant axon is shown in the bottom left corner of each panel. Scale bar = 50 μm. (B) Quantitation of ADt neuronal axon defects. Horizontal axis shows the treatment group labels and vertical axis shows the percentage of embryos in each phenotypic category (Grade 0–3) for each treatment group. Numbers inside parentheses denote numbers of animals analyzed for each treatment group. (C) Synergistic effects between sub-threshold Dcc-Netrin1 and Dcc-Neogenin morpholino knockdowns. dcc-sub_MO and ntn-sub_MO: sub-threshold concentration morpholino. At least three independent injections were performed for each treatment group. Numbers inside parentheses denote numbers of animals analyzed. Asterisks represent p<0.001 by ANOVA test.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
b1204Tg + MO1-dcc standard conditions Prim-25 dorso-rostral cluster axon extension process quality, abnormal
Prim-25 dorso-rostral cluster neuron projection mislocalised dorsally, abnormal
Prim-25 dorso-rostral cluster neuron projection physical object quality, abnormal
b1204Tg + MO1-dcc + MO1-ntn1b + MO4-ntn1a standard conditions Prim-25 dorso-rostral cluster axon extension process quality, abnormal
Prim-25 dorso-rostral cluster neuron projection mislocalised dorsally, abnormal
Prim-25 dorso-rostral cluster neuron projection physical object quality, abnormal
b1204Tg + MO1-dcc + MO2-neo1a standard conditions Prim-25 dorso-rostral cluster axon extension process quality, abnormal
Prim-25 dorso-rostral cluster neuron projection mislocalised dorsally, abnormal
b1204Tg + MO1-ntn1b standard conditions Prim-25 dorso-rostral cluster neuron projection mislocalised dorsally, normal
b1204Tg + MO1-ntn1b + MO4-ntn1a standard conditions Prim-25 dorso-rostral cluster axon extension process quality, abnormal
Prim-25 dorso-rostral cluster neuron projection mislocalised dorsally, abnormal
Prim-25 dorso-rostral cluster neuron projection physical object quality, abnormal
b1204Tg + MO2-neo1a standard conditions Prim-25 dorso-rostral cluster neuron projection mislocalised dorsally, normal
b1204Tg + MO4-ntn1a standard conditions Prim-25 dorso-rostral cluster neuron projection mislocalised dorsally, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One