ZFIN ID: ZDB-FIG-120525-27
Gao et al., 2012 - Dcc Regulates Asymmetric Outgrowth of Forebrain Neurons in Zebrafish. PLoS One   7(5):e36516 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish: b1204Tg + MO1-dcc
Knockdown Reagent: MO1-dcc
Observed In: dorso-rostral cluster axon extension, dorso-rostral cluster neuron projection
Stage: Prim-15

Fig. 3

Dcc is required for correct asymmetric outgrowth of ADt neuronal axons.

(A) Dcc expression was reduced after injection of dcc translation-blocking morpholinos into zebrafish embryos. Endogenous Dcc protein was detected as a band of approximately 170 kb. Tubulin served as a loading control. M: size marker. (B) ADt neurons project axons dorsally when Dcc function is inhibited by morpholino injection. Images of live animals were acquired as in Fig. 1C. The pixel intensity value of aberrant axon is shown in the bottom left corner of each panel. Scale bar = 50 µm. (C) Quantitation of ADt neuronal axon defects. Horizontal axis shows the treatment group labels and vertical axis shows the percentage of embryos in each phenotypic category (Grade 0–3) for each treatment group. Numbers inside parentheses denote numbers of animals analyzed for each treatment group. Asterisks and brackets represent p<0.05 by Mann-Whitney U test.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
b1204Tg + MO1-dcc standard conditions Prim-15 dorso-rostral cluster axon extension process quality, abnormal
Prim-15 dorso-rostral cluster neuron projection mislocalised dorsally, abnormal
Prim-15 dorso-rostral cluster neuron projection physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One