ZFIN ID: ZDB-FIG-111215-40
Phillips et al., 2011 - Harmonin (Ush1c) is required in zebrafish Muller glial cells for photoreceptor synaptic development and function. Disease models & mechanisms   4(6):786-800 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies: Ab-SV2, Ab1-grm
Fish: AB, AB + MO1-ush1c
Knockdown Reagent: MO1-ush1c
Anatomical Terms: retinal outer plexiform layer axon terminus, retinal outer plexiform layer dendritic spine
Stage: Day 5
PHENOTYPE:
Fish: AB + MO1-ush1c
Knockdown Reagent: MO1-ush1c
Observed In: postsynaptic density assembly, retinal cone cell cytoskeleton of presynaptic active zone, retinal outer plexiform layer postsynaptic density, retinal outer plexiform layer synapse, synapse assembly
Stage: Day 5

Fig. 7 Synaptic maturation is impaired in ush1c morphants. (A,B) Anti-SV2 (red) and -GluR2/3 (green) antibodies mark the pre-and postsynaptic regions of the OPL, respectively. Tightly associated localization of these factors is apparent in the control retina (A), and is notably disrupted in the morphant retina (B) at 5 dpf. (C–F) Electron micrographs of cone pedicles in 6 dpf control and morphant larvae. In control retinas (C,E), the presence of multiple triads (boxed areas), consisting of a synaptic ribbon (r), arciform density (arrow) and postsynaptic processes from bipolar (b) and horizontal (h) cells, indicate normal synaptic maturation. An enlargement of a triad is shown in E. In morphant retinas (D,F), fewer triads are present (boxed areas in D; enlarged in panel F), and floating ribbons (r) and distant postsynaptic processes (h) are observed in cone pedicles. Scale bars: 20 μm (A,B); 1 μm (C,D); 500 nm (E,F).

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-SV2 IHC AB standard conditions Day 5 retinal outer plexiform layer axon terminus
IHC AB + MO1-ush1c standard conditions Day 5 retinal outer plexiform layer axon terminus
Ab1-grm IHC AB standard conditions Day 5 retinal outer plexiform layer dendritic spine
IHC AB + MO1-ush1c standard conditions Day 5 retinal outer plexiform layer dendritic spine
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-ush1c standard conditions Day 5 postsynaptic density assembly disrupted, abnormal
Day 5 retinal cone cell cytoskeleton of presynaptic active zone detached from retinal outer plexiform layer postsynaptic density, abnormal
Day 5 retinal outer plexiform layer synapse disorganized, abnormal
Day 5 synapse assembly disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.