ZFIN ID: ZDB-FIG-111215-38
Phillips et al., 2011 - Harmonin (Ush1c) is required in zebrafish Muller glial cells for photoreceptor synaptic development and function. Disease models & mechanisms   4(6):786-800 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene: ush1c
Antibodies: Ab1-glul, Ab1-ush1c
Fish: AB, ush1cfh293/fh293, AB + MO1-ush1c
Knockdown Reagent: MO1-ush1c
Anatomical Terms: Muller cell, Muller cell cell projection
Stage: Day 5
PHENOTYPE:
Fish: ush1cfh293/fh293, WT + MO1-ush1c
Knockdown Reagent: MO1-ush1c
Observed In: Muller cell
Stage: Day 5

Fig. 5 Harmonin localization in the larval retina is restricted to Müller glia. Semi-thin plastic sections stained with toluidine blue show consistent size, lamination and organization of 5 dpf wild-type, mutant and morphant retinas (A,F,K). 16-μm larval retina sections (B,G,L) show colocalization of the Müller cell marker glutamine synthetase (GS; green) and anti-zebrafish harmonin (red) in wild type (B). Both antibodies label Müller cell processes throughout the retina. Anti-zebrafish harmonin labeling is reduced in mutant (G) and morphant (L) Müller cells. Müller cell number and morphology is unaffected. Nonspecific signal associated with photoreceptor outer segments is observed in all panels at this magnification. See supplementary material Fig. S4 for comparison. High-magnification images of anti-zebrafish harmonin and GS labeling in Müller cell processes at the OPL (large arrow) and OLM (small arrow) of the 5 dpf retina are shown (wild type, C–E; mutant, H–J; morphant, M–O). Scale bars: 50 μm (A,B,F,G,K,L); 20 μm (C–E,H–J,M–O).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ush1c Ab1-ush1c AB standard conditions Day 5 Muller cell IHC
Ab1-ush1c Day 5 Muller cell cell projection IHC
Ab1-ush1c AB + MO1-ush1c standard conditions Day 5 Muller cell IHC
Ab1-ush1c ush1cfh293/fh293 standard conditions Day 5 Muller cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-glul IHC AB standard conditions Day 5 Muller cell
IHC AB + MO1-ush1c standard conditions Day 5 Muller cell
IHC ush1cfh293/fh293 standard conditions Day 5 Muller cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ush1c standard conditions Day 5 Muller cell morphology, normal
ush1cfh293/fh293 standard conditions Day 5 Muller cell morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.