ZFIN ID: ZDB-FIG-111215-34
Phillips et al., 2011 - Harmonin (Ush1c) is required in zebrafish Muller glial cells for photoreceptor synaptic development and function. Disease models & mechanisms   4(6):786-800 Full text @ Dis. Model. Mech.
Anatomical Terms:
Stage: Day 5

Fig. 1 Zebrafish ush1c is expressed in retina and the inner ear. (A) The predominant splice form of ush1c corresponds to mammalian isoform A, with 21 exons that produce a 1.6 kb mRNA. A hydrophobicity plot of zebrafish (red) and human (blue) isoform A protein sequence shows conserved residue polarity. % amino acid identity with human protein is indicated below the plot. The positions of the ush1cfh293 nonsense mutation (red arrowhead), the MO target (green arrowhead) and the region recognized by the zebrafish harmonin antibody (red bar) are indicated. (B) At 5 dpf, ush1c transcripts are abundant in the inner retina (black arrow), the sensory patches of the ear (white arrows), the intestinal tract (i) and the swim bladder (sb). Fainter expression in the brain (b) and trunk muscles (m) is also observed. (C) High-magnification view of a 5 dpf whole-mount zebrafish ear showing ush1c expression in all sensory patches (ac, lc, pc: anterior, lateral and posterior cristae, respectively; am: anterior macula) and an adjacent head neuromast (n). (D) ush1c transcripts are restricted to cells in the inner nuclear layer in 5 dpf sectioned retina. (E) Central section of an adult zebrafish retina showing continued strong expression in the inner retina; expression is detected in the outer nuclear layer as well. onl, outer nuclear layer; inl, inner nuclear layer; gcl, ganglion cell layer. Scale bars: 100 μm (B); 20 μm (C); 50 μm (D,E).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ush1c AB standard conditions Day 5 anterior crista ISH
Day 5 anterior macula ISH
Day 5 brain ISH
Day 5 inner ear ISH
Day 5 intestine ISH
Day 5 lateral crista ISH
Day 5 posterior crista ISH
Day 5 retinal inner nuclear layer ISH
Day 5 retinal outer nuclear layer ISH
Day 5 swim bladder ISH
Day 5 trunk musculature ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.