ZFIN ID: ZDB-FIG-111215-37
Phillips et al., 2011 - Harmonin (Ush1c) is required in zebrafish Muller glial cells for photoreceptor synaptic development and function. Disease models & mechanisms   4(6):786-800 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene: ush1c
Antibody: Ab1-ush1c
Fish: AB, ush1cfh293/fh293, ush1cfh293/fh293 + MO1-ush1c, AB + MO1-ush1c
Knockdown Reagent: MO1-ush1c
Anatomical Terms: hair cell anterior macula cell body, hair cell anterior macula cuticular plate, hair cell anterior macula kinocilium, hair cell anterior macula stereocilium bundle
Stage: Day 5
PHENOTYPE:
Fish: ush1cfh293/fh293 + MO1-ush1c
Knockdown Reagent: MO1-ush1c
Observed In: auditory receptor cell kinocilium
Stage: Day 5

Fig. 4 Subcellular localization of harmonin in mechanosensory hair cells. Co-labeling of wild-type 5 dpf anterior macula hair cells (A–D) shows anti-zebrafish harmonin localization (red) in the hair bundles (green), kinocilia (arrowheads), cell bodies and at synaptic boutons (arrow in C and D). Incubation conditions favoring hair bundle visualization (E–P) show strong colocalization of harmonin within the bundle structure as well as in the cuticular plate in 5 dpf wild types (E–G). Kinocilia labeling can be seen above the bundle region (asterisks). Enhanced signal detection in mutant hair cells (H–J) shows localization persisting in the kinocilia and in existing bundle structures. Kinocilia are more visible in these panels owing to the reduced bundle architecture in the mutant. Protein distribution seems globular and disorganized in the cell bodies (arrowheads). Hair cells of ush1c MO larvae (K–M) show strong knockdown of harmonin, with localization persisting only in kinocilia at this stage, and no signal observed within hair bundles. Injecting the morpholino into the ush1c-/- mutants reduces subcellular localization of harmonin to undetectable levels in hair cells (N–P). Scale bars: 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ush1c Ab1-ush1c AB standard conditions Day 5 hair cell anterior macula cuticular plate IHC
Ab1-ush1c Day 5 hair cell anterior macula kinocilium IHC
Ab1-ush1c Day 5 hair cell anterior macula stereocilium bundle IHC
Ab1-ush1c AB + MO1-ush1c standard conditions Day 5 hair cell anterior macula kinocilium IHC
Ab1-ush1c ush1cfh293/fh293 standard conditions Day 5 hair cell anterior macula cell body IHC
Ab1-ush1c Day 5 hair cell anterior macula kinocilium IHC
Ab1-ush1c ush1cfh293/fh293 + MO1-ush1c standard conditions Day 5 Not Detected hair cell anterior macula IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ush1cfh293/fh293 + MO1-ush1c standard conditions Day 5 auditory receptor cell kinocilium absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.