ZFIN ID: ZDB-FIG-111215-39
Phillips et al., 2011 - Harmonin (Ush1c) is required in zebrafish Muller glial cells for photoreceptor synaptic development and function. Disease models & mechanisms   4(6):786-800 Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene: ush1c
Antibodies: Ab1-glul, Ab1-ush1c
Fish: AB
Anatomical Terms: Muller cell, Muller cell cell projection
Stage: Adult

Fig. 6 Harmonin localizes in Müller cells through all zebrafish life stages and is evolutionarily conserved. (A–F) Localization of anti-zebrafish harmonin (red) in Müller cells persists in adult zebrafish retinas. Some low level signal is sometimes observed in photoreceptor synapses (arrow in A) and in photoreceptor outer segments (arrow in D). The strongest signal within the photoreceptor layer is associated with Müller cell processes projecting into the subapical region (asterisks). Nonspecific fluorescence of photoreceptor outer segments at this stage can be compared with panel H in supplementary material Fig. S4. (G–L) Anti-mouse harmonin (SDI; G,I) and anti-human harmonin (Santa Cruz; J,L) detect localization in photoreceptors and Müller cells [glutamine synthetase (GS) is labeled in green; harmonin antibodies in red] in retinas from P10–P11 mouse pups. The outer nuclear layer (onl), inner nuclear layer (inl) and ganglion cell layer (gcl) are labeled in merged panels. Scale bars: 50 μm (A–C,G–L); 20 μm (D–F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ush1c Ab1-ush1c AB standard conditions Adult Muller cell IHC
Ab1-ush1c Adult Muller cell cell projection IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-glul IHC AB standard conditions Adult Muller cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Disease models & mechanisms for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dis. Model. Mech.