ZFIN ID: ZDB-FIG-111128-72
Muto et al., 2011 - Multifactorial Origins of Heart and Gut Defects in nipbl-Deficient Zebrafish, a Model of Cornelia de Lange Syndrome. PLoS Biology   9(10):e1001181 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Term:
Stage Range: Bud to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 2 Morpholino knock-down of Nipbls.

(A) Total protein lysates (10 embryos per lane) from uninjected embryos or embryos injected with either 0.5 ng of nipbla-MO1 or 0.5 ng of nipblb-MO1 were prepared at 10 and 24 hpf and subjected to Western blotting with anti-Nipbla and Nipblb-antibodies. Positions of Nipbla and Nipblb proteins are shown on the right. A lower band recognized by anti-Nipbla antibody (*) at 24 hpf is not reproducibly observed and may be a degradation product of Nipbla. (B) Activities of nipblb-MOs at earlier stages. EGFP reporter RNAs (100 pg/embryo)—nipbla-5′-UTR-EGFP (upper panels) and nipblb-52-UTR-EGFP (lower panels)—were injected alone or together with either nipblb-MO1 or nipblb-MO2 (1 ng/embryo), and EGFP fluorescence measured at 10–11 hpf. Views are lateral, with anterior and dorsal to the top and right, respectively. (C) Morphology of living embryos at indicated stages. Uninjected embryos, control embryos co-injected with 0.75 ng each of two 5-mis-nipbl-MOs, and embryos injected with either nipbla-MO1 (0.75 ng) or nipblb-MO1 (0.75 ng) alone or together (nipbla/b-morphants) are shown. Pericardial edema and tail defects are indicated by asterisks and arrowheads, respectively. Views are lateral, with dorsal to the top. Scale bar: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nipbla Ab1-nipbla AB standard conditions Bud whole organism WB
Ab1-nipbla Prim-5 whole organism WB
nipblb Ab1-nipblb AB standard conditions Bud whole organism WB
Ab1-nipblb Prim-5 whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-nipbla standard conditions Prim-5 pericardium edematous, abnormal
Prim-5 post-vent region decreased length, abnormal
Prim-15 blood circulation disrupted, abnormal
Prim-15 pericardium edematous, abnormal
Prim-15 post-vent region decreased length, abnormal
Long-pec post-vent region branched, abnormal
AB + MO1-nipbla + MO1-nipblb standard conditions Prim-5 pericardium edematous, abnormal
Prim-5 post-vent region decreased length, abnormal
Prim-15 pericardium edematous, abnormal
Prim-15 post-vent region decreased length, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Biol.