ZFIN ID: ZDB-FIG-111014-1
Valdivia et al., 2011 - Lef1-dependent Wnt/β-catenin signalling drives the proliferative engine that maintains tissue homeostasis during lateral line development. Development (Cambridge, England)   138(18):3931-3941 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Protruding-mouth

Fig. 1 Characterisation of the zebrafish lef1u767 mutant. (A-C) Lateral views of wild-type (A), lef1u767 (B) and lef1 morphant (C) 3 dpf zebrafish embryos stained with DiAsp/DiOC6. Arrows indicate positions of PLL neuromasts. (D) RT-PCR from sibling and lef1u767 cDNA using primers targeting exons 1 and 3. Mutant lane shows a 121 nucleotide band lacking 67 nucleotides of exon 2. DNA marker lane shows 400, 300, 200 and 100 bp bands. (E) Western blot showing absence of Lef1 band in lef1u767 mutant. (F-M) Expression of lef1 (F,G), tcf7 (H,I), tcf7l1a (J,K) and tcf7l2 (L,M) in wild-type (F,H,J,L) and lef1u767 mutant (G,I,K,M) 33 hpf embryos. Primordia are outlined. (N-Q) GFP expression in control Tg(7Xtcf-siam:GFP)ia4 Wnt reporter (N), lef1 morphant (O), BIO incubated (P) and BIO-incubated/lef1-morphant (Q) 36 hpf embryos. PLLP is outlined in each case. Brackets indicate the length of the primordium.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP ia4Tg standard conditions Prim-25 posterior lateral line primordium IFL
ia4Tg + MO3-lef1 standard conditions Prim-25 Not Detected posterior lateral line primordium IFL
lef1 WT standard conditions Prim-15 posterior lateral line primordium ISH
Protruding-mouth whole organism RTPCR
Ab1-lef1 Protruding-mouth whole organism WB
lef1u767/u767 standard conditions Prim-15 posterior lateral line primordium ISH
Protruding-mouth whole organism RTPCR
Ab1-lef1 Protruding-mouth whole organism WB
tcf7 WT standard conditions Prim-15 posterior lateral line primordium ISH
lef1u767/u767 standard conditions Prim-15 posterior lateral line primordium ISH
tcf7l1a WT standard conditions Prim-15 posterior lateral line primordium ISH
lef1u767/u767 standard conditions Prim-15 posterior lateral line primordium ISH
tcf7l2 WT standard conditions Prim-15 posterior lateral line primordium ISH
lef1u767/u767 standard conditions Prim-15 posterior lateral line primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-lef1 standard conditions Protruding-mouth posterior lateral line development decreased process quality, abnormal
Protruding-mouth posterior lateral line neuromast decreased amount, abnormal
ia4Tg + MO3-lef1 standard conditions Prim-25 Wnt signaling pathway decreased process quality, abnormal
lef1u767/u767 standard conditions Protruding-mouth posterior lateral line development decreased process quality, abnormal
Protruding-mouth posterior lateral line neuromast decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development