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ZFIN ID: ZDB-FIG-110928-15
Xie et al., 2011 - Zebrafish foxo3b Negatively Regulates Canonical Wnt Signaling to Affect Early Embryogenesis. PLoS One   6(9):e24469 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Shield
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Shield

Fig. 6

Foxo3b functions in posterior neuroectoderm formation and involves with BMP signaling in DV patterning.

(A) Foxo3b affected posterior neuroectoderm formation and mesoderm induction. A1-A2, at 80% epiboly, cdx4 expression expanded in foxo3b-MO injected embryos compared to control embryos. A3-A4, the expression of non-neural ectodermal marker gata2 was up-regulated in foxo3b-knockdown embryos. A5-A6, ectoderm marker foxi1 expanded in foxo3b morphants. (B) The expression of wnt8 and bmp ligands in foxo3b morphants. B1–B2, wnt8 expression increased in foxo3b morphants at shield stage. B3–B6, the expression of ventral markers bmp2b/bmp4 was up-regulated in foxo3b-knockdown embryos at shield stage. Embryos were injected with 8 ng STD-MO (control) or 8 ng foxo3b-ATG-MO. A1–A2, lateral views with dorsal to the right; A3–A6, animal pole views with dorsal to the right; B1–B6, lateral views with dorsal to the right; A1–A2, 80% epiboly; A3–A6, B1–B6, shield stage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp2b WT control Shield epiblast ISH
WT + MO1-foxo3b standard conditions Shield epiblast ISH
bmp4 WT control Shield epiblast ISH
WT + MO1-foxo3b standard conditions Shield epiblast ISH
cdx4 WT control Shield epiblast ISH
WT + MO1-foxo3b standard conditions Shield epiblast ISH
foxi1 WT control Shield epiblast ISH
WT + MO1-foxo3b standard conditions Shield epiblast ISH
gata2a WT control Shield epiblast ISH
WT + MO1-foxo3b standard conditions Shield epiblast ISH
wnt8a WT control Shield epiblast ISH
WT + MO1-foxo3b standard conditions Shield epiblast ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-foxo3b standard conditions Shield dorsal/ventral pattern formation disrupted, abnormal
Shield neural tube patterning disrupted, abnormal
Shield Wnt signaling pathway process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One