ZFIN ID: ZDB-FIG-110920-92
Smith et al., 2011 - Transmembrane protein 2 (Tmem2) is required to regionally restrict atrioventricular canal boundary and endocardial cushion development. Development (Cambridge, England)   138(19):4193-4198 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 4

The endocardial expansion in wkm mutants is bmp4 driven. (A-B′) ISH for zebrafish has2 in wkm mutants and siblings injected with p53 control MO (A) or p53 MO + bmp4 MO at 52 hpf and examined at 2 dpf. Dashed lines outline the morphology of the heart. Arrowhead indicates the location of the AVC. (C-F′) Endothelial Kdrl:GFP (C-D′) or endocardial Alcama (E-F′) expression in wkm mutants and siblings injected with p53 MO or p53 MO + bmp4 MO. (G) Model of AVC boundary regulation. In zebrafish, Bmp4 can drive EC primordium development in the AVC. Tmem2 restricts EC formation to the AVC by inhibiting Bmp4. A, atrium; AV, atrioventricular; EC, endocardial cushion; V, ventricle.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alcama WT + MO3-bmp4 + MO4-tp53 standard conditions Long-pec atrioventricular canal endocardium IHC
WT + MO4-tp53 standard conditions Long-pec atrioventricular canal endocardium IHC
cemip2hu4800/hu4800 + MO3-bmp4 + MO4-tp53 standard conditions Long-pec atrioventricular canal endocardium IHC
cemip2hu4800/hu4800 + MO4-tp53 standard conditions Long-pec atrioventricular canal endocardium IHC
cemip2hu5935/hu5935 + MO3-bmp4 + MO4-tp53 standard conditions Long-pec atrioventricular canal endocardium IHC
cemip2hu5935/hu5935 + MO4-tp53 standard conditions Long-pec atrioventricular canal endocardium IHC
GFP cemip2hu4800/hu4800; la116Tg + MO3-bmp4 + MO4-tp53 standard conditions Long-pec vascular endothelium IFL
cemip2hu4800/hu4800; la116Tg + MO4-tp53 standard conditions Long-pec vascular endothelium IFL
cemip2hu5935/hu5935; la116Tg + MO3-bmp4 + MO4-tp53 standard conditions Long-pec vascular endothelium IFL
cemip2hu5935/hu5935; la116Tg + MO4-tp53 standard conditions Long-pec vascular endothelium IFL
la116Tg + MO3-bmp4 + MO4-tp53 standard conditions Long-pec vascular endothelium IFL
la116Tg + MO4-tp53 standard conditions Long-pec vascular endothelium IFL
has2 WT + MO3-bmp4 + MO4-tp53 standard conditions Long-pec atrioventricular canal endocardium ISH
WT + MO4-tp53 standard conditions Long-pec atrioventricular canal endocardium ISH
cemip2hu4800/hu4800 + MO3-bmp4 + MO4-tp53 standard conditions Long-pec atrioventricular canal endocardium ISH
cemip2hu4800/hu4800 + MO4-tp53 standard conditions Long-pec atrioventricular canal endocardium ISH
cemip2hu5935/hu5935 + MO3-bmp4 + MO4-tp53 standard conditions Long-pec atrioventricular canal endocardium ISH
cemip2hu5935/hu5935 + MO4-tp53 standard conditions Long-pec atrioventricular canal endocardium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cemip2hu4800/hu4800; la116Tg + MO3-bmp4 + MO4-tp53 standard conditions Long-pec atrioventricular canal morphology, normal
Long-pec atrioventricular canal size, normal
Long-pec endocardial cushion formation process quality, normal
cemip2hu4800/hu4800; la116Tg + MO4-tp53 standard conditions Long-pec atrioventricular canal distended, abnormal
Long-pec endocardial cushion formation disrupted, abnormal
cemip2hu5935/hu5935; la116Tg + MO3-bmp4 + MO4-tp53 standard conditions Long-pec atrioventricular canal morphology, normal
Long-pec atrioventricular canal size, normal
Long-pec endocardial cushion formation process quality, normal
cemip2hu5935/hu5935; la116Tg + MO4-tp53 standard conditions Long-pec atrioventricular canal distended, abnormal
Long-pec endocardial cushion formation disrupted, abnormal
la116Tg + MO3-bmp4 + MO4-tp53 standard conditions Long-pec atrioventricular canal morphology, normal
Long-pec atrioventricular canal size, normal
Long-pec endocardial cushion formation process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development