ZFIN ID: ZDB-FIG-110920-89
Smith et al., 2011 - Transmembrane protein 2 (Tmem2) is required to regionally restrict atrioventricular canal boundary and endocardial cushion development. Development (Cambridge, England)   138(19):4193-4198 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 1

Zebrafish wkm, which is associated with abnormal cardiac looping, encodes the novel protein Tmem2. (A-B′) Bright-field images of wkm mutants and siblings. The bracket indicates pooling of blood above the ventral tail fin. (C-D′) Whole-mount in situ hybridisation (ISH) (C,C′) and sectioning (D,D′) of wkm sibling and mutant embryos for cmlc2 (myl7) and amhc (myh6) at 52 hpf. The bracket indicates elongated atrial cells in siblings but not in mutant embryonic hearts. (E-F′) ISH staining for vmhc (E,E′) and amhc (F,F′) at 52 hpf. (G,G′) Confocal z-stacks of whole-mount Alcama-immunostained (red) kdrl:GFP (green) transgenic embryos at 50 hpf. (H) Positional cloning of wkm (allele tmem2hu4800) on zebrafish chromosome 5 (Ensembl, zv8). Arrows indicate the region where the wkm mutation resides between. (I,I′) ISH for cmlc2 on embryos from a wkmhu5935 × wkmhu4800 transheterozygote cross, showing (I) unaffected and (I′) affected transheterozygotes. The number of embryos scored out of the total examined is indicated. (J) Exon sequencing revealed an A-to-T transversion (arrow) in the wkmhu5935 allele, resulting in a stop codon (K329X) in tmem2. (K) A morpholino (MO) against tmem2 (0.5 ng) reproduced the wkm phenotype in 58/81 injected embryos (72%) at 48 hpf. (L) The predicted single transmembrane domain (TM), the G8 domain and the location of the premature stop codon (star, see H) in Tmem2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alcama cemip2hu4800/hu4800; la116Tg(AB/TL) standard conditions Long-pec atrioventricular canal endocardium IHC
Long-pec myocardium IHC
cemip2hu5935/hu5935; la116Tg(AB/TL) standard conditions Long-pec atrioventricular canal endocardium IHC
Long-pec myocardium IHC
la116Tg standard conditions Long-pec atrioventricular canal endocardium IHC
Long-pec myocardium IHC
GFP cemip2hu4800/hu4800; la116Tg(AB/TL) standard conditions Long-pec endocardium IFL
cemip2hu5935/hu5935; la116Tg(AB/TL) standard conditions Long-pec endocardium IFL
la116Tg standard conditions Long-pec endocardium IFL
myh6 WT standard conditions Long-pec atrial myocardium ISH
cemip2hu4800/hu4800(AB/TL) standard conditions Long-pec atrial myocardium ISH
cemip2hu5935/hu5935(AB/TL) standard conditions Long-pec atrial myocardium ISH
myh7 WT standard conditions Long-pec ventricular myocardium ISH
cemip2hu4800/hu4800(AB/TL) standard conditions Long-pec ventricular myocardium ISH
cemip2hu5935/hu5935(AB/TL) standard conditions Long-pec ventricular myocardium ISH
myl7 WT standard conditions Long-pec heart ISH
cemip2hu4800/+; cemip2hu5935/+(AB/TL) standard conditions Long-pec heart ISH
cemip2hu4800/hu4800(AB/TL) standard conditions Long-pec heart ISH
cemip2hu5935/hu5935(AB/TL) standard conditions Long-pec heart ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-cemip2 standard conditions Long-pec heart looping disrupted, abnormal
Long-pec heart tube mislocalised radially, abnormal
cemip2hu4800/+; cemip2hu5935/+(AB/TL) standard conditions Long-pec heart looping disrupted, abnormal
Long-pec heart tube mislocalised radially, abnormal
cemip2hu4800/hu4800(AB/TL) standard conditions Long-pec atrial myocardium cardiac muscle cell shape, abnormal
Long-pec heart looping disrupted, abnormal
Long-pec heart tube mislocalised radially, abnormal
Long-pec pericardium edematous, abnormal
Long-pec post-vent region increased accumulation blood, abnormal
cemip2hu5935/hu5935(AB/TL) standard conditions Long-pec atrial myocardium cardiac muscle cell shape, abnormal
Long-pec heart looping disrupted, abnormal
Long-pec heart tube mislocalised radially, abnormal
Long-pec pericardium edematous, abnormal
Long-pec post-vent region increased accumulation blood, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development