Zebrafish cops6 is required for dorsoventral patterning and convergent extension movement in early development. cops6 morphants showed a wider expression pattern of chd, marked with arrows (A,B), but a narrower expression pattern of eve1, marked with arrows (C,D); animal pole views, dorsal towards the right at 60-75% epiboly stage. The dlx3 expression domain in (F) cops6 morphants was expanded at the 1-4 somite stage when compared to (E) control. Somite morphology of (G) wild-type and (H) cops6 morphants at the 12-14 somite stage. Lateral expansion of somite muscles was apparent (green dot lines). Furthermore, myoD expression in (J) cops6 morphants was widened and severely disrupted (red asterisk) when compared to (I) control. pax2a expression at the 12-14 somite stage (K,L) lateral view and (K–L, insert) dorsal view. Blue dotted lines represent the distance between the mid-hindbrain boundary and otic vesicles in the lateral view; while red dotted lines represent this distance in the dorsal view. Shortening of the lateral distance was found in cops6 morphants. On the other hand, white dotted lines indicated the distance between the two otic vesicles, where lengthening of the ventral distance was found in cops6 morphants. mhb: mid-hindbrain boundary; ot: otic vesicle. In all photos, the head is to the left. Scale bar: 70 μm (A-D and G-J), 25 μm (E,F) and 150 μm (K,L).
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