header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-110324-31
Sheets et al., 2011 - Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cells. Development (Cambridge, England)   138(7):1309-1319 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 7

Localization of the postsynaptic density in ribeye morphants. (A-E) Representative confocal z-projections of pan-MAGUK (green) and Ribeye a (red) immunolabel in posterior lateral line neuromast 2 of 4 dpf larvae. Scale bars: 3 μm (main panels), 1 μm (far right panels). (A,B) MAGUK immunolabel juxtaposes Ribeye-labeled punctae in wild-type (A; n=24) and ribeye b MO-injected larvae (B; n=15). Yellow pixels present in the far right panels indicate the close proximity of Ribeye to MAGUK immunolabel. The brightness of Ribeye a immunolabel in B was increased in the merged panel to better visualize its localization. (C) Ribeye immunolabel was absent in 18% (n=33) of ribeye a and ribeye b MO-injected larvae. (D) MAGUK immunolabel appeared to overlap Ribeye in 49% (n=33) of ribeye a and ribeye b MO-injected larvae. (E) MAGUK immunolabel did not overlap Ribeye in 33% (n=33) of ribeye a and ribeye b MO-injected larvae. Yellow pixels are reduced in the far right panel.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctbp2a Ab1-ctbp2a WT control Day 4 neuromast hair cell cytoskeleton of presynaptic active zone IHC
Ab1-ctbp2a WT + MO1-ctbp2a + MO2-ctbp2l standard conditions Day 4 neuromast hair cell cytoskeleton of presynaptic active zone IHC
Ab1-ctbp2a WT + MO2-ctbp2l standard conditions Day 4 neuromast hair cell cytoskeleton of presynaptic active zone IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pan-MAGUK IHC WT control Day 4 lateral line nerve postsynaptic density
IHC WT + MO1-ctbp2a + MO2-ctbp2l standard conditions Day 4 lateral line nerve postsynaptic density
IHC WT + MO2-ctbp2l standard conditions Day 4 lateral line nerve postsynaptic density
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ctbp2a + MO2-ctbp2l standard conditions Day 4 posterior lateral line nerve postsynaptic density separated from neuromast hair cell cytoskeleton of presynaptic active zone, abnormal
Day 4 postsynaptic density assembly decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development