ZFIN ID: ZDB-FIG-110324-35
Sheets et al., 2011 - Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cells. Development (Cambridge, England)   138(7):1309-1319 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 5

Fig. s1

Fig. S1. Afferent innervation and postsynaptic density localization in relation to Ribeye during zebrafish hair-cell development. Scale bars: 3 μm. (A,B) GFP-labeled innervating afferent neurons (green) and Ribeye b immunolabel (red) in a lateral-line neuromast from 2 dpf (A) and 4 dpf (B) larvae. White arrowheads in A indicate dendritic varicosities adjacent to Ribeye-positive aggregates. White arrows in A,B indicate fine dendritic processes that are not adjacent to Ribeye immunolabel. Merged images include DAPI. (C-E) Ribeye b (red) and pan-MAGUK (green) immunolabel in the developing ear at 26 hpf (C), 30 hpf (D) and 48 hpf (E). MAGUK (white arrows) is initially visible at 26 hpf, is beneath but not juxtaposed to Ribeye label at 30 hpf and is adjacent to Ribeye punctae at 48 hpf. (F) Ribeye b (red) and pan-MAGUK (green) labeling in a cross-section of a neuromast at 5 dpf. MAGUK immunolabel is juxtaposed to Ribeye labeled punctae.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctbp2l Ab3-ctbp2l WT control Prim-5 neuromast hair cell cytoskeleton of presynaptic active zone IHC
Ab3-ctbp2l Prim-15 neuromast hair cell cytoskeleton of presynaptic active zone IHC
Ab3-ctbp2l Long-pec neuromast hair cell cytoskeleton of presynaptic active zone IHC
Ab3-ctbp2l Day 5 neuromast hair cell cytoskeleton of presynaptic active zone IHC
Ab3-ctbp2l nl1Tg control Long-pec neuromast hair cell cytoskeleton of presynaptic active zone IHC
Ab3-ctbp2l Day 4 neuromast hair cell cytoskeleton of presynaptic active zone IHC
EGFP nl1Tg control Long-pec lateral line nerve dendrite IFL
Day 4 lateral line nerve dendrite IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pan-MAGUK IHC WT control Prim-5 lateral line nerve postsynaptic density
IHC Prim-15 lateral line nerve postsynaptic density
IHC Long-pec lateral line nerve postsynaptic density
IHC Day 5 lateral line nerve postsynaptic density
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development