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ZFIN ID: ZDB-FIG-110324-26
Sheets et al., 2011 - Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cells. Development (Cambridge, England)   138(7):1309-1319 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Day 5

Fig. 1

ribeye a and ribeye b expression and localization during zebrafish hair-cell development. (A) Expression of ribeye a and ribeye b mRNA in the developing ear and neuromasts. ribeye b is expressed strongly in the otic vesicle at 20 hpf, while ribeye a is expressed after 20 hpf, and at lower levels (black arrowheads). At 52 hpf, ribeye a is detectable in the retina and ear (black arrowhead), whereas ribeye b is expressed in both the ear (black arrowhead) and lateral line neuromasts (black and white arrowheads). Scale bar: 200 μm. (B-E) Representative maximum confocal z-projections of hair cells in wild-type larvae. Scale bars: 3 ┬Ám. (B-D) Ribeye immunofluorescent labeling in nascent hair cells in the developing ear at 22 hpf (B), 26 hpf (C) and 28 hpf (D). Ribeye a and Ribeye b proteins form aggregates that, by 26 hpf, are accumulated at the basal end of hair cells (white arrowheads). (E) Ribeye immunofluorescent labeling in a cross-section of a neuromast at 5 dpf. Merged images include DAPI labeling of cell nuclei (blue).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ctbp2a WT standard conditions 20-25 somites Not Detected otic vesicle ISH
Ab1-ctbp2a 26+ somites inner ear hair cell IHC
Ab1-ctbp2a Prim-5 inner ear hair cell IHC
Prim-5 otic vesicle ISH
Long-pec inner ear ISH
Long-pec retina ISH
Ab1-ctbp2a Day 5 inner ear hair cell IHC
ctbp2l WT standard conditions 20-25 somites otic vesicle ISH
Ab3-ctbp2l 26+ somites inner ear hair cell IHC
Ab3-ctbp2l Prim-5 inner ear hair cell IHC
Prim-5 otic vesicle ISH
Long-pec inner ear ISH
Long-pec neuromast ISH
Ab3-ctbp2l Day 5 inner ear hair cell IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development