header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-110324-27
Sheets et al., 2011 - Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cells. Development (Cambridge, England)   138(7):1309-1319 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec

Fig. 2

Morpholino-mediated knockdown of ribeye a and ribeye b in 2-day-old zebrafish larvae. (A-D) Representative confocal z-projections of Ribeye a (red) and Ribeye b (green) immunolabel in neuromast hair cells of wild-type (A), ribeye a morphant (B), ribeye b morphant (C), and ribeye a and ribeye b morphant larvae (D). Merged images include DAPI (blue). Scale bar: 3 μm. E) The average number of Ribeye a punctae per neuromast (white circles) in control (11±2) and ribeye b MO-injected (10±2) larvae, and the average number of Ribeye b punctae (black circles) in control (7±1) and ribeye a MO-injected (9±2) larvae. Each circle represents neuromast 1 in the trunk of an individual larva. Error bars indicate s.e.m. (F) Total pixel intensity (A.U.) of Ribeye a immunolabel in control and ribeye b morphants (Mann-Whitney U-test: P<0.0001), and Ribeye b in control and ribeye a morphants (Mann-Whitney U-test: P=0.80). Whiskers indicate the 10th and 90th percentiles. (G) RT-PCR analysis of control (C) and antisense ribeye b splice site MO-injected whole larvae. cav1.3a was used as a control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cacna1da WT control Long-pec whole organism RTPCR
WT + MO2-ctbp2l standard conditions Long-pec whole organism RTPCR
ctbp2a Ab1-ctbp2a WT control Long-pec neuromast hair cell IHC
Long-pec whole organism RTPCR
Ab1-ctbp2a WT + MO1-ctbp2a standard conditions Long-pec Not Detected neuromast hair cell IHC
Ab1-ctbp2a WT + MO1-ctbp2a + MO2-ctbp2l standard conditions Long-pec Not Detected neuromast hair cell IHC
Ab1-ctbp2a WT + MO2-ctbp2l standard conditions Long-pec neuromast hair cell IHC
Long-pec whole organism RTPCR
ctbp2l Ab3-ctbp2l WT control Long-pec neuromast hair cell IHC
Long-pec whole organism RTPCR
Ab3-ctbp2l WT + MO1-ctbp2a standard conditions Long-pec neuromast hair cell IHC
Ab3-ctbp2l WT + MO1-ctbp2a + MO2-ctbp2l standard conditions Long-pec Not Detected neuromast hair cell IHC
Ab3-ctbp2l WT + MO2-ctbp2l standard conditions Long-pec Not Detected neuromast hair cell IHC
Long-pec Not Detected whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development