ZFIN ID: ZDB-FIG-110324-29
Sheets et al., 2011 - Ribeye is required for presynaptic CaV1.3a channel localization and afferent innervation of sensory hair cells. Development (Cambridge, England)   138(7):1309-1319 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig. 5

Depletion of Ribeye b results in a reduction in the number of spikes in afferent neurons. (A) Extracellular action currents recorded from a posterior lateral line neuron in response to 20 Hz mechanical stimulation of neuromast hair cells (4 dpf). Shown are overlays of 60 consecutive sweeps from wild-type (black circle) and three ribeye b MO-injected larvae with variable degrees of knockdown (see Results; grey circle, blue circle and red circle). (B) Four frequency histograms (20 Hz) for all spikes recorded in A. Numbers above each histogram indicate the total number of spikes. The overall width and shape of each histogram illustrates that the degree of phase-locking with the stimulus was maintained despite the reduction in spike number. (C) Confocal z-projections of neuromasts from a representative wild-type larva and from the three neuromasts recorded from the ribeye b MO-injected larvae in A. The left panels show Ribeye a staining (red) and the innervating afferent neuron labeled with GFP (green). The right panels show corresponding Ribeye b staining (green). In a larva that was responsive to acoustic stimuli yet displayed a mild balance defect (grey circle), Ribeye b levels were less depleted than the two larvae unresponsive to acoustic stimuli (blue and red circles). Scale bar: 3 μm. Asterisks indicate the tract of the lateral-line nerve. (D) Plot of the relationship between spikes per deflection and the number of Ribeye b punctae. Each circle represents a recorded neuromast from an individual morphant larva, except for the black circle, which represents the average of several wild-type larvae, including that shown in A. The grey, blue and red circles are from the three individual neuromasts in A-C. Error bars indicate s.e.m.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ctbp2a Ab1-ctbp2a nl1Tg control Day 4 neuromast hair cell cytoskeleton of presynaptic active zone IHC
Ab1-ctbp2a nl1Tg + MO2-ctbp2l standard conditions Day 4 neuromast hair cell cytoskeleton of presynaptic active zone IHC
ctbp2l Ab3-ctbp2l nl1Tg control Day 4 neuromast hair cell cytoskeleton of presynaptic active zone IHC
Ab3-ctbp2l nl1Tg + MO2-ctbp2l standard conditions Day 4 neuromast hair cell cytoskeleton of presynaptic active zone IHC
EGFP nl1Tg control Day 4 lateral line nerve dendrite IFL
nl1Tg + MO2-ctbp2l standard conditions Day 4 lateral line nerve dendrite IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nl1Tg + MO2-ctbp2l standard conditions Day 4 detection of mechanical stimulus involved in sensory perception of sound decreased process quality, abnormal
Day 4 neuromast hair cell cytoskeleton of presynaptic active zone decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development