ZFIN ID: ZDB-FIG-110111-32
Gallardo et al., 2010 - Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish. BMC Developmental Biology   10:120 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Prim-25
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-25

Fig. 4 Validation of microarray analysis. (A) Quantitative RT-PCR for individual genes with different biological roles was performed of RNA derived from GFP+ and GFP- cells from tails of 36 hpf embryos. Real time PCR ratios were determined by normalization to β-actin (equal to 1, dotted line). Only 3 genes out of the 15 tested were not significantly enriched (asterisks). (B-G) In situ hybridization of 6 genes enriched in GFP+ cells showing a primordium specific expression pattern in 36 hpf embryos. (H-O) Loss-of-function analysis on two selected genes (cd9b and f11r) enriched in GFP+ cells of 36 hpf embryos. Cldnb:gfp embryos were injected with anti-sense morpholinos (MO) against cd9b (I, L-M) and f11r (J, N-O), and compared to control (H, K). White arrows indicate the position where the primordium is at 36 hpf (H-J). Red arrowheads indicate the rosette-like structures in the primordium (K). Scale bars are 10 μm in (B-G) and (K-O).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cd9b WT standard conditions Prim-25 posterior lateral line primordium ISH
cd99 WT standard conditions Prim-25 posterior lateral line primordium ISH
cldn7b WT standard conditions Prim-25 posterior lateral line primordium ISH
dkk1b WT standard conditions Prim-25 posterior lateral line primordium ISH
f11r.1 WT standard conditions Prim-25 posterior lateral line primordium ISH
ndr1 WT standard conditions Prim-25 posterior lateral line primordium ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf106Tg + MO1-cd9b standard conditions Prim-25 posterior lateral line primordium organization quality, abnormal
Prim-25 posterior lateral line primordium shape, abnormal
zf106Tg + MO1-f11r.1 standard conditions Prim-25 posterior lateral line primordium organization quality, abnormal
Prim-25 posterior lateral line primordium shape, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.